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Literature summary for 3.4.22.16 extracted from
- Separation of a new alpha-N-benzoylarginine-beta-naphthylamide hydrolase from cathepsin B1 (1978), J. Biol. Chem., 253, 4319-4326.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | required | Oryctolagus cuniculus |  |
| thiol compounds | required | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
gel filtration | Oryctolagus cuniculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Proteins + H2O | Oryctolagus cuniculus | aminopeptidase and endopeptidase activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
6 isoenzymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| lung | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryctolagus cuniculus |
| 7.45 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Na acetate buffer, pH 4.6, 1 mM EDTA, 1 month, concentrated preparations, no loss of activity | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O | - |
Oryctolagus cuniculus | alpha-N-benzoyl-DL-arginine + beta-naphthylamine | - |
ir | |
| Leu-2-naphthylamide + H2O | - |
Oryctolagus cuniculus | Leu + 2-naphthylamine | - |
ir | |
| Proteins + H2O | aminopeptidase and endopeptidase activity | Oryctolagus cuniculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-N-benzoylarginine-beta-naphthylamide hydrolase | - |
Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
substrate Leu-2-naphthylamide | Oryctolagus cuniculus |
| 49 | - |
substrate benzoyl-Arg 2-naphthylamide | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
substrate benzoyl-Arg 2-naphthylamide | Oryctolagus cuniculus |
| 7.2 | - |
substrate Leu-2-naphthylamide | Oryctolagus cuniculus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
little sensitivity to alkaline pH | Oryctolagus cuniculus |
| 8.5 | - |
83% activity | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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