Application | Comment | Organism |
---|---|---|
medicine | the enzyme is a target for vaccine and chemotherapy for controlling Fasciola gigantica infection | Fasciola gigantica |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain GS115 | Fasciola gigantica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38300 | - |
x * 38300, deglycosylated pro-form, SDS-PAGE | Fasciola gigantica |
40700 | - |
x * 40700, activated mature enzyme in glycosylated form, SDS-PAGE | Fasciola gigantica |
47600 | - |
x * 47600, glycosylated pro-form, SDS-PAGE | Fasciola gigantica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
immunoglobulin G + H2O | Fasciola gigantica | - |
? | - |
? | |
Laminin + H2O | Fasciola gigantica | - |
? | - |
? | |
Type I collagen + H2O | Fasciola gigantica | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola gigantica | Q6T857 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Fasciola gigantica |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA affinity column chromatography | Fasciola gigantica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
digestive tract | - |
Fasciola gigantica | - |
epithelial cell | - |
Fasciola gigantica | - |
juvenile | - |
Fasciola gigantica | - |
juvenile | newly excysted | Fasciola gigantica | - |
metacercaria | - |
Fasciola gigantica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | preferred substrate | Fasciola gigantica | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
immunoglobulin G + H2O | - |
Fasciola gigantica | ? | - |
? | |
Laminin + H2O | - |
Fasciola gigantica | ? | - |
? | |
Type I collagen + H2O | - |
Fasciola gigantica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38300, deglycosylated pro-form, SDS-PAGE | Fasciola gigantica |
? | x * 40700, activated mature enzyme in glycosylated form, SDS-PAGE | Fasciola gigantica |
? | x * 47600, glycosylated pro-form, SDS-PAGE | Fasciola gigantica |
Synonyms | Comment | Organism |
---|---|---|
CatL1H | - |
Fasciola gigantica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Fasciola gigantica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | more than 50% activity between pH 5.5 and 8.0 | Fasciola gigantica |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a role in host tissue migration and immune evasion | Fasciola gigantica |