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Literature summary for 3.4.22.15 extracted from

  • Sansri, V.; Changklungmoa, N.; Chaichanasak, P.; Sobhon, P.; Meemon, K.
    Molecular cloning, characterization and functional analysis of a novel juvenile-specific cathepsin L of Fasciola gigantica (2013), Acta Trop., 128, 76-84.
    View publication on PubMed
Search for more literature for 3.4.22.15

Application

Application Comment Organism
medicine the enzyme is a target for vaccine and chemotherapy for controlling Fasciola gigantica infection Fasciola gigantica

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Fasciola gigantica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38300
-
 x * 38300, deglycosylated pro-form, SDS-PAGE Fasciola gigantica
40700
-
 x * 40700, activated mature enzyme in glycosylated form, SDS-PAGE Fasciola gigantica
47600
-
 x * 47600, glycosylated pro-form, SDS-PAGE Fasciola gigantica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
immunoglobulin G + H2O Fasciola gigantica
-
 ?
-
 ?
Laminin + H2O Fasciola gigantica
-
 ?
-
 ?
Type I collagen + H2O Fasciola gigantica
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fasciola gigantica Q6T857
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Fasciola gigantica

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA affinity column chromatography Fasciola gigantica

Source Tissue

Source Tissue Comment Organism Textmining
digestive tract
-
 Fasciola gigantica
-
epithelial cell
-
 Fasciola gigantica
-
juvenile
-
 Fasciola gigantica
-
juvenile newly excysted Fasciola gigantica
-
metacercaria
-
 Fasciola gigantica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Fasciola gigantica benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O preferred substrate Fasciola gigantica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
immunoglobulin G + H2O
-
 Fasciola gigantica ?
-
 ?
Laminin + H2O
-
 Fasciola gigantica ?
-
 ?
Type I collagen + H2O
-
 Fasciola gigantica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38300, deglycosylated pro-form, SDS-PAGE Fasciola gigantica
? x * 40700, activated mature enzyme in glycosylated form, SDS-PAGE Fasciola gigantica
? x * 47600, glycosylated pro-form, SDS-PAGE Fasciola gigantica

Synonyms

Synonyms Comment Organism
CatL1H
-
 Fasciola gigantica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Fasciola gigantica

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 more than 50% activity between pH 5.5 and 8.0 Fasciola gigantica

General Information

General Information Comment Organism
physiological function the enzyme plays a role in host tissue migration and immune evasion Fasciola gigantica