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Literature summary for 3.4.22.15 extracted from

  • Janoir, C.; Pechine, S.; Grosdidier, C.; Collignon, A.
    Cwp84, a surface-associated protein of Clostridium difficile, is a cysteine protease with degrading activity on extracellular matrix proteins (2007), J. Bacteriol., 189, 7174-7180.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.15

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol proteolytic activity requires presence of dithiothreitol. Autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol Clostridioides difficile

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridioides difficile

Inhibitors

Inhibitors Comment Organism Structure
E64 0.1 mM, complete inhibition Clostridioides difficile
EDTA 5 mM, 81% residual activity Clostridioides difficile
iodoacetic acid 10 mM, 15% residual activity Clostridioides difficile
leupeptin 0.1 mM, less than 10% residual activity Clostridioides difficile
N-ethylmaleimide 10 mM, less than 10% residual activity Clostridioides difficile
pepstatin 0.01 mM, 90% residual activity Clostridioides difficile
phenylmethylsulfonyl fluoride 10 mM, less than 10% residual activity Clostridioides difficile
TLCK 10 mM, less than 10% residual activity Clostridioides difficile

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information enzyme is found to be associated with the S-layer proteins, mostly as a nonmature species Clostridioides difficile
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE Clostridioides difficile
68000
-
 x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile Q83UF3
-
-
Clostridioides difficile 79-685 Q83UF3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol or trypsin Clostridioides difficile

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridioides difficile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibronectin + H2
-
 Clostridioides difficile ? enzyme cleaves fibronectin to produce two bands ?
fibronectin + H2
-
 Clostridioides difficile 79-685 ? enzyme cleaves fibronectin to produce two bands ?
laminin + H2
-
 Clostridioides difficile ?
-
 ?
laminin + H2
-
 Clostridioides difficile 79-685 ?
-
 ?
additional information proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen Clostridioides difficile ?
-
 ?
additional information proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen Clostridioides difficile 79-685 ?
-
 ?
vitronectin + H2
-
 Clostridioides difficile ?
-
 ?
vitronectin + H2
-
 Clostridioides difficile 79-685 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE Clostridioides difficile

Synonyms

Synonyms Comment Organism
Cwp84
-
 Clostridioides difficile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Clostridioides difficile

pH Range

pH Minimum pH Maximum Comment Organism
3.5 8 more than 80% of maximum activity Clostridioides difficile
10
-
 30% of maximum activity Clostridioides difficile

pI Value

Organism Comment pI Value Maximum pI Value
Clostridioides difficile isoelectric focusing
-
 7.5