Crystallization (Comment) | Organism |
---|---|
the inactive C25A cathepsin L mutant is cocrystallized with a histone H3 peptide covering residues 19-33 of the human histone H3 tail (QLATKAARKSAPATG), sitting drop vapor diffusion method, using 10% (v/v) isopropanol, 0.1 M sodium acetate trihydrate, pH 4.0, 22% polyethylene glycol 6000. The final crystallization condition for the apo-mC25A crystals is 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate, pH 4.6, 30% (w/v) polyethylene glycol 2000 monomethylether | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C25A | inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KAPR-acetyl-K-QLATKAARKSAPA | - |
Homo sapiens | |
KAPRKQLAT-acetyl-K-AARKSAPA | - |
Homo sapiens | |
KAPRKQLATKAAR-dimethyl-K-SAPA | - |
Homo sapiens | |
KAPRKQLATKAARKSAPA | - |
Homo sapiens | |
L-trans-epoxysuccinylleucylamido-(4-guanidino)butane | i.e. E-64, complete inhibition at 0.26 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000037 | - |
Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
0.000063 | - |
Abz-PRKQLATKAARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
0.000073 | - |
Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
0.00016 | - |
Abz-PR-acetyl-K-QLATKAARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3 + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07711 | - |
- |
Purification (Comment) | Organism |
---|---|
CM Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-PR-acetyl-K-QLATKAARKSAK-Dnp + H2O | - |
Homo sapiens | ? | - |
? | |
Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp + H2O | - |
Homo sapiens | ? | - |
? | |
Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp + H2O | - |
Homo sapiens | ? | - |
? | |
Abz-PRKQLATKAARKSAK-Dnp + H2O | - |
Homo sapiens | ? | - |
? | |
histone H3 + H2O | - |
Homo sapiens | ? | - |
? | |
L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-Leu-L-Arg + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
0.46 | - |
Abz-PRKQLATKAARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
0.49 | - |
Abz-PR-acetyl-K-QLATKAARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
0.51 | - |
Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp | pH and temperature not specified in the publication | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
KAPRKQLATKAARKSAPA | pH and temperature not specified in the publication | Homo sapiens | |
0.00455 | - |
KAPRKQLATKAAR-dimethyl-K-SAPA | pH and temperature not specified in the publication | Homo sapiens | |
0.00502 | - |
KAPRKQLAT-acetyl-K-AARKSAPA | pH and temperature not specified in the publication | Homo sapiens | |
0.0107 | - |
KAPR-acetyl-K-QLATKAARKSAPA | pH and temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2900 | - |
Abz-PR-acetyl-K-QLATKAARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
5300 | - |
Abz-PRKQLATKAAR-dimethyl-K-SAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
6100 | - |
Abz-PRKQLATKAARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens | |
6700 | - |
Abz-PRKQLAT-acetyl-K-AARKSAK-Dnp | pH and temperature not specified in the publication | Homo sapiens |