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Literature summary for 3.4.22.15 extracted from

  • Costa, T.F.; Reis, F.C.; Lima, A.P.
    Substrate inhibition and allosteric regulation by heparan sulfate of Trypanosoma brucei cathepsin L (2012), Biochim. Biophys. Acta, 1824, 493-501.
    View publication on PubMed
Search for more literature for 3.4.22.15

Activating Compound

Activating Compound Comment Organism Structure
heparin sulfate heparin sulfate (0.02 mg/ml) has no detectable effect on the rate of hydrolysis of the substrate at 0.0025 mM while it increases by 10fold the rate of substrate hydrolysis at 0.05 mM Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
L-Phe-L-Arg-7-amido-4-methylcoumarin the susceptibility of recombinant enzyme to substrate inhibition at 25°C is partially reversible, i.e., it is absent at 37°C and is displayed when the same enzyme sample is assayed again at 25°C Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00033
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C Trypanosoma brucei
0.00037
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.01 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.00041
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.005 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.00044
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.00046
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.00047
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.02 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.00695
-
 L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C Trypanosoma brucei
0.01242
-
 L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fibrinogen + H2O Trypanosoma brucei
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibrinogen + H2O
-
 Trypanosoma brucei ?
-
 ?
L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma brucei L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma brucei L-Val-L-Leu-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
CatL
-
 Trypanosoma brucei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 37 the activity of CATL decays at substrate concentrations higher than 0.01 mM, in a concentration-dependent manner. The activity of CATL is unaltered at saturation concentrations of substrate when the protease is assayed at 37°C Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.01 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.43
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.02 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.45
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.005 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.52
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.52
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
0.89
-
 L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
1.01
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C Trypanosoma brucei
1.07
-
 L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05241
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
71.6
-
 L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
155.3
-
 L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C Trypanosoma brucei
999.8
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.02 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
1088
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.01 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
1119
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
1121
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in the presence of 0.005 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
1187
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C Trypanosoma brucei
3148
-
 L-Phe-L-Arg-7-amido-4-methylcoumarin in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C Trypanosoma brucei