Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymostatin | 32.24% residual activity at 0.2 mM | Dosidicus gigas | |
E-64 | i.e. L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane, 24.76% residual activity at 1 mg/ml | Dosidicus gigas | |
leupeptin | 11.06% residual activity at 0.001 mg/ml | Dosidicus gigas | |
pefabloc | 78.43% residual activity at 2 mg/ml | Dosidicus gigas | |
pepstatin | 58.06% residual activity at 0.001 mg/ml | Dosidicus gigas | |
Soybean trypsin inhibitor | 63.12% residual activity at 0.05 mg/ml | Dosidicus gigas | |
tosyl phenylalanyl chloromethylketone | 66.63% residual activity at 0.1 mg/ml | Dosidicus gigas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23790 | - |
MALDI-TOF mass spectrometry | Dosidicus gigas |
24000 | - |
SDS-PAGE | Dosidicus gigas |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | Dosidicus gigas | - |
? | - |
? | |
casein + H2O | Dosidicus gigas | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dosidicus gigas | - |
jumbo squid | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, column chromatography, Toyopearl HW-55 F gel filtration | Dosidicus gigas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Dosidicus gigas | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the crude extract has a specific activity of 110 units/mg protein, the 15fold purified enzyme has a specific activity of 1644 units/mg protein at pH 4.5 and 55°C, one enzyme unit is defined as the absorbance at 380 nm given by 0.001 mM of free 7-amino-4-methylcoumarin per min | Dosidicus gigas |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Dosidicus gigas | ? | - |
? | |
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin + H2O | cathepsin L specific synthetic substrate | Dosidicus gigas | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-trifluoromethylcoumarin | - |
? | |
casein + H2O | - |
Dosidicus gigas | ? | - |
? | |
additional information | the cathepsin B and H specific synthetic substrates benzyloxycarbonyl-L-Ala-L-Ala-7-amido-4-trifluoromethylcoumarin and L-Arg-7-amido-4-methylcoumarin do not show any hydrolysis with the partially purified enzyme | Dosidicus gigas | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24000, SDS-PAGE | Dosidicus gigas |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin | Dosidicus gigas |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | about 60% of the maximum activity at 40°C and at 70°C with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin | Dosidicus gigas |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin | Dosidicus gigas |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 6 | about 70% of the maximum activity at pH 3.5 and about 45% of the maximum activity at pH 6.0 with the substrate benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-trifluoromethylcoumarin | Dosidicus gigas |