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Literature summary for 3.4.22.15 extracted from

  • Lecaille, F.; Chowdhury, S.; Purisima, E.; Broemme, D.; Lalmanach, G.
    The S2 subsites of cathepsins K and L and their contribution to collagen degradation (2007), Protein Sci., 16, 662-670.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.15

Cloned(Commentary)

Cloned (Comment) Organism
mutants expressed in Pichia pastoris Homo sapiens

Protein Variants

Protein Variants Comment Organism
A205L mutant shows an effective cathepsin K-like preference for Leu and Pro Homo sapiens
L67Y/A205L mutation induces a switch of the enzymatic specificity toward small selective inhibitors and peptidyl substrates Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Homo sapiens
N-(4-biphenylacetyl)-S-methylcysteine-(D)-Arg-Phe-beta-phenethylamide also called Cat L inhibitor 7, specific inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme L67Y/A205L Homo sapiens
0.0013
-
 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens
0.0087
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens
0.0098
-
 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin mutant enzyme L67Y/A205L Homo sapiens
0.014
-
 benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin mutant enzyme L67Y/A205L Homo sapiens
0.081
-
 benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
butyl-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Collagen + H2O substrate for cathepsin L mutant A205L Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens
0.5
-
 benzyloxycarbonyl-Gly-Pro-Arg-7-amido-4-methylcoumarin mutant enzyme L67Y/A205L Homo sapiens
5.4
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens
17.6
-
 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin wild type enzyme Homo sapiens
20.5
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin mutant enzyme L67Y/A205L Homo sapiens
27.2
-
 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin mutant enzyme L67Y/A205L Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0043
-
 N-(4-biphenylacetyl)-S-methylcysteine-(D)-Arg-Phe-beta-phenethylamide wild type enzyme Homo sapiens