Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 172% activity in the presence of 2 mM dithiothreitol and 1 mM EDTA, dithiothreitol alone does not inhibit activity | Hypophthalmichthys molitrix | |
L-cysteine | 114% activity at 5 mM | Hypophthalmichthys molitrix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 50% activity at 2 mM | Hypophthalmichthys molitrix | |
Ca2+ | 51% residual activity at 1 mM | Hypophthalmichthys molitrix | |
Co2+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
Cu2+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
E-64 | complete inhibition at 0.1 mM | Hypophthalmichthys molitrix | |
EDTA | 98% residual activity at 2 mM | Hypophthalmichthys molitrix | |
Fe2+ | 5% residual activity at 1 mM | Hypophthalmichthys molitrix | |
Fe3+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
Mg2+ | 66% residual activity at 1 mM | Hypophthalmichthys molitrix | |
Mn2+ | 65% residual activity at 1 mM | Hypophthalmichthys molitrix | |
additional information | insensitive to K+, phenylmethylsulfonylfluoride and pepstatin A | Hypophthalmichthys molitrix | |
Ni2+ | complete inhibition at 1 mM | Hypophthalmichthys molitrix | |
Zn2+ | 10% residual activity at 1 mM | Hypophthalmichthys molitrix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00825 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Hypophthalmichthys molitrix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Hypophthalmichthys molitrix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hypophthalmichthys molitrix | - |
silver carp | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephacel gel filtration, Sephacryl S-100 gel filtration, SP Sepharose fast flow chromatography, and Con A Sepharose chromatography | Hypophthalmichthys molitrix |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Hypophthalmichthys molitrix | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
crude extract | Hypophthalmichthys molitrix |
6 | - |
after 48.4fold purification | Hypophthalmichthys molitrix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O | 7% activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Hypophthalmichthys molitrix | benzyloxycarbonyl-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | 9% activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Hypophthalmichthys molitrix | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | 100% activity | Hypophthalmichthys molitrix | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
surimi protein + H2O | - |
Hypophthalmichthys molitrix | ? | - |
? | |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O | 1% activity compared to benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Hypophthalmichthys molitrix | tert-butyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin L1 | - |
Hypophthalmichthys molitrix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Hypophthalmichthys molitrix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | the enzyme exhibits a higher hydrolytic activity on surimi protein at 65°C than at 40°C | Hypophthalmichthys molitrix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | - |
Hypophthalmichthys molitrix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Hypophthalmichthys molitrix |