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Literature summary for 3.4.22.15 extracted from

  • Dufour, E.; Obled, A.; Valin, C.; Bechet, D.; Ribadeau-Dumas, B.; Huet, J.C.
    Purification and amino acid sequence of chicken liver cathepsin L (1987), Biochemistry, 26, 5689-5695.
    View publication on PubMed
Search for more literature for 3.4.22.15

Activating Compound

Activating Compound Comment Organism Structure
thiol-reducing agent required for hydrolysis of benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
Benzyloxycarbonyl-Phe-Phe-CHN2
-
 Gallus gallus
leupeptin
-
 Gallus gallus
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Gallus gallus 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23668
-
 x * 23668, calculation from amino acid composition Gallus gallus
27000
-
 x * 27000, SDS-PAGE under reducing conditions Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2500
-
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Gallus gallus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE under reducing conditions Gallus gallus
? x * 23668, calculation from amino acid composition Gallus gallus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide
-
 Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Gallus gallus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4
-
 2 min, 97% loss of activity Gallus gallus