Any feedback?
Literature summary for 3.4.22.14 extracted from
- Anionic proteinase from Actinidia chinensis. Preparation and properties of the crystalline enzyme (1970), Eur. J. Biochem., 14, 214-221.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Actinidia chinensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4,4'-dithiodipyridine | - |
Actinidia chinensis |  |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Actinidia chinensis | |
| iodoacetate | - |
Actinidia chinensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 89 | - |
benzoyl-L-Arg ethyl ester | pH 5.6, 25°C, in 0.3 M KCl | Actinidia chinensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12800 | - |
gel filtration | Actinidia chinensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinidia chinensis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Actinidia chinensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Actinidia chinensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crystalline enzyme, resuspended in dialysis buffer at pH 4, stable for several months | Actinidia chinensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-L-Arg ethyl ester + H2O | - |
Actinidia chinensis | Benzoyl-L-Arg + ethanol | - |
? | |
| Gelatin + H2O | - |
Actinidia chinensis | ? | - |
? | |
| p-toluene-sulphonyl-L-glutamine p-nitrophenyl ester + H2O | - |
Actinidia chinensis | p-toluene-sulphonyl-L-glutamine + 4-nitrophenol | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
benzoyl-L-Arg ethyl ester | pH 5.6, 25°C, in 0.3 M KCl | Actinidia chinensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | - |
Actinidia chinensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8.8 | about 45% of maximal activity at pH 3.5 and at pH 8.8 | Actinidia chinensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
