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Literature summary for 3.4.22.14 extracted from

  • Sugiyama, S.; Ohtsuki, K.; Sato, K.; Kawabata, M.
    Purification and characterization of six kiwifruit proteases isolated with two ion-exchange resins, Toyopearl-Super Q and Bakerbond WP-PEI (1996), Biosci. Biotechnol. Biochem., 60, 1994-2000.
No PubMed abstract available
Search for more literature for 3.4.22.14

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activates Actinidia chinensis

Inhibitors

Inhibitors Comment Organism Structure
1-(L-trans-epoxysuccinylleucylamino)-4-guanidinobutane
-
 Actinidia chinensis
Cd2+
-
 Actinidia chinensis
Cu2+
-
 Actinidia chinensis
Hg2+
-
 Actinidia chinensis
leupeptin
-
 Actinidia chinensis
PCMB
-
 Actinidia chinensis
Zn2+
-
 Actinidia chinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23500
-
 x * 23500, SDS-PAGE Actinidia chinensis

Organism

Organism UniProt Comment Textmining
Actinidia chinensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
actinidin can be separated into six proteases, named KP1, KP2, KP3, KP4, KP5 and KP6 Actinidia chinensis

Source Tissue

Source Tissue Comment Organism Textmining
fruit juice Actinidia chinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
 isoenzyme KP2 Actinidia chinensis
0.034
-
 isoenzyme KP3 Actinidia chinensis
0.04
-
 isoenzyme KP1 Actinidia chinensis
0.051
-
 isoenzyme KP5 Actinidia chinensis
0.177
-
 isoenzyme KP6 Actinidia chinensis
0.197
-
 isoenzyme KP4 Actinidia chinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinylcasein + H2O
-
 Actinidia chinensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 23500, SDS-PAGE Actinidia chinensis