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Literature summary for 3.4.22.14 extracted from
- Proteolytic activities of kiwifruit actinidin (Actinidia deliciosa cv. Hayward) on different fibrous and globular proteins: a comparative study of actinidin with papain (2014), Appl. Biochem. Biotechnol., 172, 4025-4037.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0582 | - |
N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester | pH 6.5, 27°C, Vmax: 2.14 miroM/sec | Actinidia deliciosa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinidia deliciosa | - |
- |
- |
| Actinidia deliciosa cv. Hayward | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | efficiently hydrolized in acidic pHs | Actinidia deliciosa | ? | - |
? | |
| casein + H2O | efficiently hydrolized in acidic pHs | Actinidia deliciosa cv. Hayward | ? | - |
? | |
| Collagen + H2O | efficiently hydrolized at neutral or mild basic pH | Actinidia deliciosa | ? | - |
? | |
| Collagen + H2O | efficiently hydrolized at neutral or mild basic pH | Actinidia deliciosa cv. Hayward | ? | - |
? | |
| Fibrinogen + H2O | efficiently hydrolized at neutral or mild basic pH | Actinidia deliciosa | ? | - |
? | |
| Fibrinogen + H2O | efficiently hydrolized at neutral or mild basic pH | Actinidia deliciosa cv. Hayward | ? | - |
? | |
| N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O | - |
Actinidia deliciosa | N-alpha-benzyloxycarbonyl-L-lysine + 4-nitrophenol | - |
? | |
| N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester + H2O | - |
Actinidia deliciosa cv. Hayward | N-alpha-benzyloxycarbonyl-L-lysine + 4-nitrophenol | - |
? | |
| sheep IgG + H2O | actinidin is ineffective on the heavy chain but has limited effects on the light chain | Actinidia deliciosa | ? | - |
? | |
| sheep IgG + H2O | actinidin is ineffective on the heavy chain but has limited effects on the light chain | Actinidia deliciosa cv. Hayward | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| actinidin | - |
Actinidia deliciosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Actinidia deliciosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 42.8 | - |
N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester | pH 6.5, 27°C | Actinidia deliciosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
efficient proteolytic activity towards casein | Actinidia deliciosa |
| 6.5 | - |
assay at | Actinidia deliciosa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 730 | - |
N-alpha-benzyloxycarbonyl-L-lysine 4-nitrophenyl ester | pH 6.5, 27°C | Actinidia deliciosa | |
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Release 2023.1 (January 2023)
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