KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
N-acetyl-L-Phe-Gly methyl ester | above 0.25 mM, at 4.3% acetonitrile, 25°C, pH 5.3, parameter for pre-steady-state acylation phase | Actinidia chinensis | |
0.543 | - |
N-acetyl-L-Phe-Gly methyl ester | 25°C, pH 5.3, 8.3% acetonitrile parameter for pre-steady-state acylation phase | Actinidia chinensis | |
0.893 | - |
N-acetyl-L-Phe-Gly methyl ester | 25°C, pH 5.3, 16.7% acetonitrile, parameter for pre-steady-state acylation phase | Actinidia chinensis |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics. With low acetonitrile content, plots of reaction are linear, increasing the acetonitrile content results in marked deviations of the plots from linearity with a rate minimum around [S]0 of 0.15 mM | Actinidia chinensis |
dimethyl sulfoxide | comparison of effect on reaction kinetics with acetonitrile | Actinidia chinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinidia chinensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-L-Phe-Gly methyl ester + H2O | stopped-flow spectral analysis of increasing content of co-solvent acetonitrile on kinetics | Actinidia chinensis | ? | - |
? |