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Literature summary for 3.4.22.10 extracted from

  • Doran, J.D.; Nomizu, M.; Takebe, S.; Menard, R.; Griffith, D.; Ziomek, E.
    Autocatalytic processing of the streptococcal cysteine protease zymogen: processing mechanism and characterization of the autoproteolytic cleavage sites (1999), Eur. J. Biochem., 263, 145-151.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, the zymogen proSCP is secreted to the Escherichia coli periplasm followed by subsequent cleavage of the signal peptide Streptococcus pyogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.193
-
benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide
-
Streptococcus pyogenes

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
-
-
Streptococcus pyogenes B220
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification autocatalytic maturation of the zymogen proceeds through the sequential appearance of at least six intermediates, resulting from cleavage after Lys26, Asn41, Lys101, Ala112 and Lys118 Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-aminobenzoyl-Ala-Ala-Ile-Lys-Ala-Gly-Ala-Arg + H2O
-
Streptococcus pyogenes ?
-
?
3-aminobenzoyl-Ala-Ala-Ile-Lys-Ala-Gly-Ala-Arg + H2O
-
Streptococcus pyogenes B220 ?
-
?
3-aminobenzoyl-Ala-Glu-Ile-Lys-Gln-Pro-Val-Val + H2O
-
Streptococcus pyogenes ?
-
?
3-aminobenzoyl-Asp-Lys-Val-Asn-Leu-Gly-Gly-Glu + H2O
-
Streptococcus pyogenes ?
-
?
3-aminobenzoyl-Asp-Lys-Val-Asn-Leu-Gly-Gly-Glu + H2O
-
Streptococcus pyogenes B220 ?
-
?
3-aminobenzoyl-Glu-Gln-Ile-Lys-Glu-Asn-Lys-Lys + H2O
-
Streptococcus pyogenes ?
-
?
3-aminobenzoyl-Glu-Gln-Ile-Lys-Glu-Asn-Lys-Lys + H2O
-
Streptococcus pyogenes B220 ?
-
?
3-aminobenzoyl-Thr-Thr-Thr-Ala-Gly-Thr-Ala-Glu + H2O
-
Streptococcus pyogenes ?
-
?
3-aminobenzoyl-Thr-Thr-Thr-Ala-Gly-Thr-Ala-Glu + H2O
-
Streptococcus pyogenes B220 ?
-
?
benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide + H2O
-
Streptococcus pyogenes ?
-
?
benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide + H2O
-
Streptococcus pyogenes B220 ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
benzyloxycarbonyl-Val-Arg-4-methylcoumarinyl-7-amide
-
Streptococcus pyogenes