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Literature summary for 3.4.22.10 extracted from

  • Watts, A.B.; Brocklehurst, K.
    Streptopain (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1245-1249.
No PubMed abstract available
Search for more literature for 3.4.22.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant C47S Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant mutant C47S, X-ray diffraction crystal structure determination and analysis at 1.6 A resolution Streptococcus pyogenes

Protein Variants

Protein Variants Comment Organism
C47S crystal structure analysis Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 40000, immature zymogen, x * 28000, mature processed enzyme Streptococcus pyogenes
40000
-
 x * 40000, immature zymogen, x * 28000, mature processed enzyme Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pyogenes streptopain shows strepadhesin activity, independent of protease activity, mediated by a cell surface adhesin and controlled by the multiple gene regulator Mga ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pyogenes
-
 the enzyme is expressed in all strains
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme performs autolytic processing to the mature 28 kDa protein by removal of an about 117 amino-acid peptide from the N-terminus Streptococcus pyogenes

Reaction

Reaction Comment Organism Reaction ID
preferential cleavage with hydrophobic residues at P2, P1 and P1' the catalytic residue is His195 forming an ion pair with the single Cys47 residue Streptococcus pyogenes
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibronectin + H2O
-
 Streptococcus pyogenes ?
-
 ?
human interleukin 1beta precursor + H2O
-
 Streptococcus pyogenes human interleukin 1beta + human interleukin 1beta signal peptide
-
 ?
insulin beta chain + H2O reduced and carboxylated substrate, cleavage of the linkage Phe25-Tyr26 Streptococcus pyogenes ?
-
 ?
additional information streptopain shows strepadhesin activity, independent of protease activity, mediated by a cell surface adhesin and controlled by the multiple gene regulator Mga Streptococcus pyogenes ?
-
 ?
additional information cleavage site specificity, overview, the enzyme prefers cleavage of Phe-Tyr bonds, the enzyme shows poor activity with trypsin substrates fibrin, casein, and gelatin from human and rabbit Streptococcus pyogenes ?
-
 ?
Vitronectin + H2O
-
 Streptococcus pyogenes ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, immature zymogen, x * 28000, mature processed enzyme Streptococcus pyogenes
More primary, and tertiary structure Streptococcus pyogenes

Synonyms

Synonyms Comment Organism
interleukin-1beta convertase
-
 Streptococcus pyogenes
More the enzyme belongs to the peptidase family C10 Streptococcus pyogenes
pyrogenic exotoxin B
-
 Streptococcus pyogenes
SpeB
-
 Streptococcus pyogenes
Streptococcal cysteine proteinase
-
 Streptococcus pyogenes
streptococcal proteinase
-
 Streptococcus pyogenes
Streptococcus peptidase A
-
 Streptococcus pyogenes