Literature summary for 3.4.22.10 extracted from

Anderson, E.T.; Winter, L.A.; Fernsten, P.; Olmsted, S.B.; Matsuka, Y.V.
The pro-sequence domain of streptopain directs the folding of the mature enzyme (2005), Arch. Biochem. Biophys., 436, 297-306.

Search for more literature for 3.4.22.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression of 12 kDa pro-sequence domain of the enzyme in Escherichia coli, expression of mature enzyme in Escherichia coli in inclusion bodies	Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment)	Organism
analysis of a crystal structure model of the zymogen	Streptococcus pyogenes

Protein Variants

Protein Variants	Comment	Organism
C192S	inactive mutant	Streptococcus pyogenes

Inhibitors

Inhibitors	Comment	Organism	Structure
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane	i.e. E64	Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
12000	-	pro-sequence, gel filtration	Streptococcus pyogenes
28000	-	mature enzyme, gel filtration	Streptococcus pyogenes

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pyogenes	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the enzyme performs autolytic processing to the 28 kDa mature protein, the 12 kDa pro-sequence domain, residues 28-145, directs the folding of the mature enzyme, overview	Streptococcus pyogenes

Purification (Commentary)

Purification (Comment)	Organism
recombinant pro-sequence domain and refolded mature enzyme from Escherichia coli by affinity chromatography	Streptococcus pyogenes

Reaction

Reaction	Comment	Organism	Reaction ID
preferential cleavage with hydrophobic residues at P2, P1 and P1'	catalytic residue Cys192	Streptococcus pyogenes	a

Renatured (Commentary)

Renatured (Comment)	Organism
refolding of denatured recombinant mature wild-type enzyme from inclusion bodies in 100 mM Na-acetate, pH 4.5, 8 M urea, 10 mM DTT, and 280 mM NaCl, by rapid dilution 1:20 into phosphate buffered saline, pH 7.4, with 10 mM DTT	Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme performs autolytic processing to the mature protein, interaction overview	Streptococcus pyogenes	?	-	?
resorufin-labeled casein + H2O	-	Streptococcus pyogenes	?	-	?

Subunits

Subunits	Comment	Organism
More	the recombinant 12 kDa pro-sequence domain of the enzyme directs the folding of the mature enzyme, interaction overview	Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Streptococcus pyogenes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Streptococcus pyogenes

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Release 2023.1 (January 2023)