Application | Comment | Organism |
---|---|---|
pharmacology | Nicotiana benthamiana has recently emerged as a versatile expression platform for the rapid generation of recombinant biopharmaceuticals, but product yield and quality frequently suffer from unintended proteolysis. Targeted downregulation of cathepsin B can improve the performance of this plant-based expression platform | Nicotiana benthamiana |
Cloned (Comment) | Organism |
---|---|
heterologous expression of NbCathB in insect cells | Nicotiana benthamiana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
Abz-GIVRAK-Dnp | 37°C, pH 4.5 | Nicotiana benthamiana | |
0.0014 | - |
Abz-FRF(NO2)A | 37°C, pH 4.5 | Nicotiana benthamiana | |
0.048 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Nicotiana benthamiana | |
0.099 | - |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Nicotiana benthamiana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana benthamiana | Q1HER6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the theoretical molecular mass of pro-NbCathB is 36700 Da, whereas that of the mature protease is 28600 Da | Nicotiana benthamiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-FRF(NO2)A + H2O | - |
Nicotiana benthamiana | ? | - |
? | |
Abz-GIVRAK-Dnp + H2O | - |
Nicotiana benthamiana | ? | - |
? | |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Nicotiana benthamiana | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Nicotiana benthamiana | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
human anti-HIV-1 antibody + H2O | NbCathB is far more efficient than aleurain-like protease in processing the human anti-HIV-1 antibody 2F5 into fragments | Nicotiana benthamiana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NbCathB | - |
Nicotiana benthamiana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Nicotiana benthamiana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Abz-GIVRAK-Dnp | 37°C, pH 4.5 | Nicotiana benthamiana | |
2.9 | - |
Abz-FRF(NO2)A | 37°C, pH 4.5 | Nicotiana benthamiana | |
4.7 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Nicotiana benthamiana | |
11 | - |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Nicotiana benthamiana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Nicotiana benthamiana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
98 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Nicotiana benthamiana | |
111 | - |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Nicotiana benthamiana | |
116 | - |
Abz-GIVRAK-Dnp | 37°C, pH 4.5 | Nicotiana benthamiana | |
2037 | - |
Abz-FRF(NO2)A | 37°C, pH 4.5 | Nicotiana benthamiana |