Cloned (Comment) | Organism |
---|---|
ProFgCB5 is expressed as a recombinant, N-terminal His-tagged protein in Escherichia coli M15. Expression in Pichia pastoris | Fasciola gigantica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystatin C | - |
Fasciola gigantica | |
stefin-1 | - |
Fasciola gigantica | |
stefin-2 | - |
Fasciola gigantica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2622 | - |
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | 37°C, pH 4.5 | Fasciola gigantica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola gigantica | A0A0U4HGR1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cecal epithelium | expressed in later juvenile stages and the mature worm | Fasciola gigantica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Fasciola gigantica | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | the enzyme shows substantial exopeptidase activity at a relative rate of 34.26% compared to human cathepsin B | Fasciola gigantica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cathepsin B5 | - |
Fasciola gigantica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Fasciola gigantica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4.5 | - |
maximal stability | Fasciola gigantica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Fasciola gigantica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | pH 3.0: about75 % of maximal activity, pH 6.0: about 50% of maximal activity | Fasciola gigantica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00013 | - |
37°C, pH 4.5 | Fasciola gigantica | stefin-2 | |
0.000486 | - |
37°C, pH 4.5 | Fasciola gigantica | cystatin C | |
0.00068 | - |
37°C, pH 4.5 | Fasciola gigantica | stefin-1 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme could be stable and highly active in the endolysosomal pathway degrading endocytosed peptides predigested by cathepsin L in the gut lumen whereas as a secreted protease it might be short lived but still stimulating an immune response. Due to its exopeptidase activity it could be involved in the regulative processing of other proteins. It is not involved in the immediate infection process | Fasciola gigantica |