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Literature summary for 3.4.22.1 extracted from

  • Sun, L.; Zhu, B.; Wu, H.; Yu, L.; Zhou, D.; Dong, X.; Yang, J.; Li, D.; Ye, W.; Murata, Y.
    Purification and characterization of cathepsin B from the gut of the sea cucumber (Stichopus japonicas) (2011), Food Sci. Biotechnol., 20, 919-925.
No PubMed abstract available
Search for more literature for 3.4.22.1

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 77.3% residual activity at 1 mM Apostichopus japonicus
antipain complete inhibition at 1 mM Apostichopus japonicus
Cu2+ 32% residual activity at 1 mM Apostichopus japonicus
E-64 complete inhibition at 1 mM Apostichopus japonicus
Fe2+ 79.9% residual activity at 1 mM Apostichopus japonicus
Fe3+ 82.3% residual activity at 1 mM Apostichopus japonicus
iodoacetic acid complete inhibition at 1 mM Apostichopus japonicus
leupeptin 42.5% residual activity at 0.1 mM Apostichopus japonicus
Mn2+ 85.4% residual activity at 1 mM Apostichopus japonicus
additional information not inhibited by dithiothreitol, L-cysteine, EDTA, and EGTA Apostichopus japonicus
Ni2+ 26.7% residual activity at 1 mM Apostichopus japonicus
phenylmethylsulfonyl fluoride 62.8% residual activity at 1 mM Apostichopus japonicus
Soybean trypsin inhibitor 51.2% residual activity at 0.1 g/l Apostichopus japonicus
Zn2+ 14.7% residual activity at 1 mM Apostichopus japonicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ a slight increase of activity (4.2%) is induced by 1 mM Ca2+ Apostichopus japonicus
Mg2+ a slight increase of activity (3.3%) is induced by 1 mM Mg2+ Apostichopus japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 1 * 26000 + 1 * 23000, SDS-PAGE Apostichopus japonicus
26000
-
 1 * 26000 + 1 * 23000, SDS-PAGE Apostichopus japonicus

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, DEAE Sepharose CL-6B column chromatography, Sephadex G-75 gel filtration, and TSK-Gel 3000 SWxl gel filtration Apostichopus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
 Apostichopus japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.19
-
 crude extract, at pH 5.5 and 45°C Apostichopus japonicus
96.7
-
 after 81.26fold purification, at pH 5.5 and 45°C Apostichopus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Apostichopus japonicus N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-carbobenzoxy-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Apostichopus japonicus N-carbobenzoxy-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 26000 + 1 * 23000, SDS-PAGE Apostichopus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Apostichopus japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 about 80% activity after 10 min at 45°C, the enzyme activity remains stable for 20 min at 45°C, and decreases with the incubation time extending to 70 min, with a 50% activity loss Apostichopus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Apostichopus japonicus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 the protease activity is stable in the acidic range between pH 4.5 and 6.0, and declines above pH 6.5 rapidly. At pH 6.5, more than 60% of the enzyme activity is lost Apostichopus japonicus