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Literature summary for 3.4.22.1 extracted from

  • Valadares, N.F.; Dellamano, M.; Soares-Costa, A.; Henrique-Silva, F.; Garratt, R.C.
    Molecular determinants of improved cathepsin B inhibition by new cystatins obtained by DNA shuffling (2010), BMC Struct. Biol., 10, 30.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
canecystatin-1
-
Homo sapiens
canecystatin-4
-
Homo sapiens
additional information not inhibited by oryzacystatin-1 N-terminal deletion, reverse mutant 1 (T30I), reverse mutant 2 (Q97L), and reverse mutant 3 (T30I, Q97L) Homo sapiens
oryzacystatin-1
-
Homo sapiens
oryzacystatin-1 clone A10
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
Homo sapiens L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000058
-
canecystatin-4 in 100 mM sodium acetate pH 5.5, 2.5 mM dithiothreitol, at 37°C Homo sapiens
0.0000112
-
oryzacystatin-1 clone A10 in 100 mM sodium acetate pH 5.5, 2.5 mM dithiothreitol, at 37°C Homo sapiens
0.0000785
-
oryzacystatin-1 in 100 mM sodium acetate pH 5.5, 2.5 mM dithiothreitol, at 37°C Homo sapiens
0.0000876
-
canecystatin-1 in 100 mM sodium acetate pH 5.5, 2.5 mM dithiothreitol, at 37°C Homo sapiens