Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for therapeutic inhibitors or vaccination | Fasciola hepatica |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged pro-catB1 in Pichia pastoris | Fasciola hepatica |
expression of mutant enzymes in Pichia pastoris | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H110A/S115A | site-directed mutagenesis, the S115A mutation eliminates an O-glycosylation site | Homo sapiens |
S115A | site-directed mutagenesis, the S115A mutation eliminates an O-glycosylation site | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CA-074Me | - |
Fasciola hepatica | |
CA-074Me | - |
Homo sapiens | |
E-64 | an irreversible cysteine protease inhibitor | Fasciola hepatica | |
E-64 | an irreversible cysteine protease inhibitor | Homo sapiens | |
human cystatin A | - |
Fasciola hepatica | |
human cystatin A | - |
Homo sapiens | |
human cystatin B | - |
Fasciola hepatica | |
Human cystatin C | - |
Fasciola hepatica | |
Human cystatin C | - |
Homo sapiens | |
additional information | no inhibition by human cystatin B | Homo sapiens | |
sheep cystatin B | - |
Fasciola hepatica | |
sheep cystatin B | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
N-benzyloxycarbonyl-Val-Ile-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.047 | - |
N-benzyloxycarbonyl-Phe-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
0.06 | - |
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.1 | - |
N-benzyloxycarbonyl-Arg-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
0.19 | - |
N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.35 | - |
Boc-Asp-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.53 | - |
Boc-Val-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.88 | - |
Tos-Gly-Pro-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
1.16 | - |
Boc-Asp-Pro-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
1.22 | - |
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
1.67 | - |
Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
4.53 | - |
D-Val-Leu-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Fasciola hepatica | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fasciola hepatica | - |
metacercariae are propagated and harvested from the snail species Lymnaea tormentosa | - |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant catB1 is mixed glycosylated | Fasciola hepatica |
proteolytic modification | the recombinant pro-catB1 is autoprocessed | Fasciola hepatica |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged pro-catB1 from Pichia pastoris by nickel affinity chromatography | Fasciola hepatica |
recombinant mutant enzymes from Pichia pastoris | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
Fasciola hepatica | - |
additional information | liver fluke at excysted juvenile stage | Fasciola hepatica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-Asp-Pro-Arg-4-methyl-7-amidocoumarin + H2O | - |
Homo sapiens | Boc-Asp-Pro-Arg + 4-methyl-7-aminocoumarin | - |
? | |
Boc-Asp-Pro-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | Boc-Asp-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin + H2O | - |
Homo sapiens | Boc-Val-Leu-Lys + 4-methyl-7-aminocoumarin | - |
? | |
Boc-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | Boc-Val-Pro-Arg + 7-amino-4-methylcoumarin | - |
? | |
D-Val-Leu-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | D-Val-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | Boc-Val-Leu-Lys-4-methylcoumarin 7-amide is no substrate of catB1. CatB1 substrate specificity, homology modelling and molecular dynamics simulations of FhcatB1 interaction with peptide substrates, molecular modelling, overview. A P2 Ile residue positions the substrate optimally for interaction with catalytic residues of the enzyme, and the enzyme lacks an occluding loop His residue crucial for exopeptidase activity. The enzyme performs autolytic processing | Fasciola hepatica | ? | - |
? | |
additional information | No activity with Boc-Val-Pro-Arg-4-methyl-7-amidocoumarin, Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin, and D-Val-Leu-Arg-4-methyl-7-amidocoumarin. Wild-type cathepsin B exhibits exopeptidase activity | Homo sapiens | ? | - |
? | |
N-benzyloxycarbonyl-Arg-Arg-4-methyl-7-amidocoumarin + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-Arg-Arg + 4-methyl-7-aminocoumarin | - |
? | |
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Phe-Arg-4-methyl-7-amidocoumarin + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-Phe-Arg + 4-methyl-7-aminocoumarin | - |
? | |
N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Val-Ile-Arg-4-methyl-7-amidocoumarin + H2O | - |
Homo sapiens | N-benzyloxycarbonyl-Val-Ile-Arg + 4-methyl-7-aminocoumarin | - |
? | |
N-benzyloxycarbonyl-Val-Ile-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | N-benzyloxycarbonyl-Val-Ile-Arg + 7-amino-4-methylcoumarin | - |
? | |
Tos-Gly-Pro-Arg-4-methyl-7-amidocoumarin + H2O | - |
Homo sapiens | Tos-Gly-Pro-Arg + 4-methyl-7-aminocoumarin | - |
? | |
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O | - |
Fasciola hepatica | Tos-Gly-Pro-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CatB1 | - |
Fasciola hepatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Fasciola hepatica |
22 | - |
hydrolytic assay and autoprocessing assay at room temperature | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.13 | - |
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.14 | - |
D-Val-Leu-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.19 | - |
N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.28 | - |
Boc-Asp-Pro-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
0.51 | - |
N-benzyloxycarbonyl-Val-Ile-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.69 | - |
Boc-Asp-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.72 | - |
Boc-Val-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
1.09 | - |
N-benzyloxycarbonyl-Arg-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
1.71 | - |
N-benzyloxycarbonyl-Phe-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
2.26 | - |
Tos-Gly-Pro-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
3.38 | - |
Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
autoprocessing | Fasciola hepatica |
5.5 | 7 | catB1shows optimal activity within this range | Fasciola hepatica |
6 | - |
hydrolytic assay at | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | FhcatB1is active across a broad pH range, approximately 30-80% of maximal activity at pH 5.0 and pH 8.0 | Fasciola hepatica |
4 | 8.5 | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rates of association of cysteine protease inhibitors with catB1, overview | Fasciola hepatica | |
0.000093 | - |
Human cystatin C | pH 6.0, 22°C | Fasciola hepatica | |
0.000215 | - |
sheep cystatin B | pH 6.0, 22°C | Fasciola hepatica | |
0.000225 | - |
human cystatin A | pH 6.0, 22°C | Fasciola hepatica | |
0.0022 | - |
human cystatin B | pH 6.0, 22°C | Fasciola hepatica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000006 | - |
pH 6.0, 22°C | Homo sapiens | Human cystatin C | |
0.00037 | - |
pH 6.0, 22°C | Homo sapiens | human cystatin A | |
0.00041 | - |
pH 6.0, 22°C | Homo sapiens | sheep cystatin B |
General Information | Comment | Organism |
---|---|---|
physiological function | the FhcatB1 protease functions largely as a digestive enzyme in the gut of the parasite. It may be likely important to a vital stage of the parasite's life cycle | Fasciola hepatica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
D-Val-Leu-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
0.244 | - |
Boc-Asp-Pro-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
1.046 | - |
N-benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
1.293 | - |
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
1.346 | - |
Boc-Val-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
1.976 | - |
Boc-Asp-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
2.2 | - |
Boc-Val-Leu-Lys-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
2.227 | - |
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
2.56 | - |
Tos-Gly-Pro-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
7.104 | - |
N-benzyloxycarbonyl-Val-Ile-Arg-4-methylcoumarin 7-amide | pH 6.0, 22°C | Fasciola hepatica | |
10.54 | - |
N-benzyloxycarbonyl-Arg-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens | |
36.59 | - |
N-benzyloxycarbonyl-Phe-Arg-4-methyl-7-amidocoumarin | pH 6.0, 22°C | Homo sapiens |