Cloned (Comment) | Organism |
---|---|
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-2 in insoluble inclusion bodies in Escherichia coli | Necator americanus |
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-3 in soluble, secreted form in Pichia pastoris strain X-33 | Necator americanus |
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-4 in insoluble inclusion bodies in Escherichia coli | Necator americanus |
quantitation of cysteine protease gene expression in different developmental stages, sequence comparison and phylogenetic analysis, overview. Expression of Na-CP-5 in insoluble inclusion bodies in Escherichia coli | Necator americanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 55000, proisozyme Na-CP-3, SDS-PAGE, x * 30000, about, processed isozyme Na-CP-3, SDS-PAGE | Necator americanus |
55000 | - |
x * 55000, proisozyme Na-CP-3, SDS-PAGE, x * 30000, about, processed isozyme Na-CP-3, SDS-PAGE | Necator americanus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Necator americanus | - |
cathepsin B-like cysteine protease Na-CP-2 | - |
Necator americanus | - |
cathepsin B-like cysteine protease Na-CP-3 | - |
Necator americanus | - |
cathepsin B-like cysteine protease Na-CP-4 | - |
Necator americanus | - |
cathepsin B-like cysteine protease Na-CP-5 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Na-CP-2 N-glycosylation site at position 140 | Necator americanus |
glycoprotein | Na-CP-3 N-glycosylation site at positions 32, 136, 242, and 298 | Necator americanus |
glycoprotein | Na-CP-4 N-glycosylation site at positions 134 and 296 | Necator americanus |
glycoprotein | Na-CP-5 N-glycosylation site at positions 101, 135 and 245 | Necator americanus |
proteolytic modification | Na-CP-3 autoactivates by proteolytic cleavage at pH 4.0 | Necator americanus |
Purification (Comment) | Organism |
---|---|
recombinant isozyme Na-CP-2 from Escherichia coli by denaturation and by nickel-NTA affinity chromatography under denaturing conditions | Necator americanus |
recombinant isozyme Na-CP-4 from Escherichia coli by denaturation and by nickel-NTA affinity chromatography under denaturing conditions | Necator americanus |
recombinant isozyme Na-CP-5 from Escherichia coli by denaturation and by nickel-NTA affinity chromatography under denaturing conditions | Necator americanus |
recombinant Na-CP-3 from yeast cell culture supernatant by nickel affinity chromatography | Necator americanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | Na-CP-2 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm | Necator americanus | - |
gut | Na-CP-3 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm | Necator americanus | - |
gut | Na-CP-4 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm | Necator americanus | - |
gut | Na-CP-5 is up-regulated during the transition from a free-living larva to a blood-feeding adult worm | Necator americanus | - |
additional information | developmental expression pattern, the isozyme is highly up-regulated in the adult stage, overview | Necator americanus | - |
additional information | developmental expression patterns, the isozymes are highly up-regulated in the adult stage, overview | Necator americanus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbobenzoxy-Phe-Arg-7-amido-4-methylcoumarin + H2O | a fluorogenic substrate of Na-CP-3 | Necator americanus | carbobenzoxy-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
Gelatin + H2O | a substrate of Na-CP-3 | Necator americanus | ? | - |
? | |
additional information | no activity with carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin by Na-CP-3, recombinant Na-CP-3 does not digest intact hemoglobin but digests globin fragments generated by prior hydrolysis with aspartic hemoglobinases | Necator americanus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, proisozyme Na-CP-3, SDS-PAGE, x * 30000, about, processed isozyme Na-CP-3, SDS-PAGE | Necator americanus |
Synonyms | Comment | Organism |
---|---|---|
CatB | - |
Necator americanus |
cathepsin B-like cysteine protease | - |
Necator americanus |
Na-CP-2 | - |
Necator americanus |
Na-CP-3 | - |
Necator americanus |
Na-CP-4 | - |
Necator americanus |
Na-CP-5 | - |
Necator americanus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | recombinant isozyme Na-CP-3 | Necator americanus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | recombinant isozyme Na-CP-3 | Necator americanus |