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Literature summary for 3.4.22.1 extracted from

  • Hamer, I.; Delaive, E.; Dieu, M.; Abdel-Sater, F.; Mercy, L.; Jadot, M.; Arnould, T.
    Up-regulation of cathepsin B expression and enhanced secretion in mitochondrial DNA-depleted osteosarcoma cells (2009), Biol. Cell, 101, 31-41.
    View publication on PubMed
Search for more literature for 3.4.22.1

Cloned(Commentary)

Cloned (Comment) Organism
expression analysis by realtime PCR, regulation of enzyme expression takes place at the transcriptional level, transcriptional transactivation of the NF-kappaB-responsive promoter which is higher in cells with mitochondria depleted in mtDNA Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular cathepsin B is secreted as an inactive precursor form, up-regulation of cathepsin B expression and enhanced secretion in mitochondrial DNA-depleted osteosarcoma cells resulting, at least in part, from the activation of the transcription factor NF-kappaB Homo sapiens
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-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 39000, enzyme precursor form, SDS-PAGE, x * 30000-31000, intermediate single-chain enzyme form, SDS-PAGE, x* 24000-25000, mature double-chain enzyme form, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens NF-kappaB induced up-regulation of cathepsin B expression contributes to the invasive property of the 143B cells lacking mtDNA, overview. Extracellularly released cathepsin B can act alone or in concert with some matrix metalloproteinases ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cathepsin B is secreted as an inactive precursor form and activated by cleavage through pepsin at pH 3.5 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
143B cell
-
 Homo sapiens
-
osteosarcoma cell up-regulation of cathepsin B expression and enhanced secretion in mitochondrial DNA-depleted osteosarcoma cells resulting, at least in part, from the activation of the transcription factor NF-kappaB Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NF-kappaB induced up-regulation of cathepsin B expression contributes to the invasive property of the 143B cells lacking mtDNA, overview. Extracellularly released cathepsin B can act alone or in concert with some matrix metalloproteinases Homo sapiens ?
-
 ?
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, enzyme precursor form, SDS-PAGE, x * 30000-31000, intermediate single-chain enzyme form, SDS-PAGE, x* 24000-25000, mature double-chain enzyme form, SDS-PAGE Homo sapiens
More peptide mapping in 143B cells by 2D gel electrophoresis and mass spectrometry Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 assay at Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens multiple forms of cathepsin B derived peptides with pIs ranging from a pI of 5.5 to a pI of 5.9 5.9 5.5