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Literature summary for 3.4.22.1 extracted from
- Catalytic and physico-chemical characteristics of goat spleen cathepsin B (1997), Biochem. Mol. Biol. Int., 42, 1215-1226.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Capra hircus |  |
| cysteamine | activates | Capra hircus | |
| cysteine | activates | Capra hircus | |
| dithiothreitol | activates | Capra hircus | |
| glutathione | activates | Capra hircus | |
| thioglycerol | activates | Capra hircus | |
| thiol compounds | required for full activity | Capra hircus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-tosylamide-2-phenylethyl chloromethylketone | moderate effect | Capra hircus | |
| antipain | - |
Capra hircus | |
| Hg2+ | - |
Capra hircus | |
| iodoacetamide | - |
Capra hircus | |
| iodoacetic acid | - |
Capra hircus | |
| L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane | E-64 | Capra hircus | |
| leupeptin | - |
Capra hircus | |
| Mn2+ | - |
Capra hircus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00145 | - |
hemoglobin | - |
Capra hircus | |
| 0.058 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | Capra hircus | |
| 0.262 | - |
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide | benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin | Capra hircus | |
| 0.685 | - |
benzoyl-DL-Arg-p-nitroanilide | - |
Capra hircus | |
| 2.5 | - |
benzoyl-DL-Arg-beta-naphthylamide | - |
Capra hircus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | 28000 | gel filtration, SDS-PAGE | Capra hircus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Proteins + H2O | Capra hircus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capra hircus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | 6.3% carbohydrate | Capra hircus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Capra hircus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Capra hircus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.247 | - |
benzoyl-DL-Arg-p-nitroanilide as substrate | Capra hircus |
| 0.562 | - |
benzoyl-DL-Arg-beta-naphthylamide | Capra hircus |
| 0.65 | - |
benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide | Capra hircus |
| 1.13 | - |
benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide | Capra hircus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Capra hircus | benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
| benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Capra hircus | benzoyl-DL-Arg + p-nitroaniline | - |
? | |
| benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Capra hircus | benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
| benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Capra hircus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
| casein + H2O | - |
Capra hircus | hydrolyzed casein | - |
? | |
| Hemoglobin + H2O | - |
Capra hircus | ? | - |
? | |
| Proteins + H2O | - |
Capra hircus | ? | - |
? | |
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