Any feedback?
Literature summary for 3.4.22.1 extracted from
- Purification and properties of brain cathepsin B. Evidence for cleavage of pituitary lipotropins (1979), Eur. J. Biochem., 101, 23-30.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activates | Bos taurus |  |
| cysteine | activates | Bos taurus | |
| dithiothreitol | activates | Bos taurus | |
| EDTA | activates | Bos taurus | |
| thiol compounds | absolute requirement | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Bos taurus | |
| chymostatin | 80% inhibition at 6 microM | Bos taurus | |
| Hg2+ | - |
Bos taurus | |
| iodoacetic acid | - |
Bos taurus | |
| leupeptin | - |
Bos taurus | |
| Zn2+ | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.53 | - |
benzoyl-DL-Arg-beta-naphthylamide | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27500 | - |
gel filtration, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Proteins + H2O | Bos taurus | turnover of intracellular proteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cerebral cortex | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Bos taurus | benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
| beta-lipotropin + H2O | - |
Bos taurus | hydrolyzed beta-lipotropin | - |
? | |
| histones + H2O | - |
Bos taurus | hydrolyzed histones | - |
? | |
| Proteins + H2O | turnover of intracellular proteins | Bos taurus | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
benzoyl-DL-Arg-beta-naphthylamide | - |
Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bos taurus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
