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Literature summary for 3.4.22.1 extracted from
- Purification and some properties of buffalo liver cathepsin B (1986), J. Biochem., 100, 293-299.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | activates | Bubalus bubalis |  |
| dithiothreitol | activates | Bubalus bubalis | |
| EDTA | activates | Bubalus bubalis | |
| thiol compounds | required for full activity | Bubalus bubalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.82 | - |
benzoyl-DL-Arg-beta-naphthylamide | - |
Bubalus bubalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27300 | 28300 | gel filtration, SDS-PAGE | Bubalus bubalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Proteins + H2O | Bubalus bubalis | turnover of intracellular proteins | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bubalus bubalis | - |
buffalo | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bubalus bubalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bubalus bubalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.71 | - |
benzoyl-DL-Arg-beta-naphthylamide as substrate | Bubalus bubalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Bubalus bubalis | benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
| benzoyl-DL-Arg-p-nitroanilide + H2O | - |
Bubalus bubalis | benzoyl-DL-Arg + p-nitroaniline | - |
? | |
| denatured hemoglobin + H2O | - |
Bubalus bubalis | hydrolyzed denatured hemoglobin | - |
? | |
| Proteins + H2O | turnover of intracellular proteins | Bubalus bubalis | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bubalus bubalis |
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