Literature summary for 3.4.21.B6 extracted from

Shipway, A.; Danahay, H.; Williams, J.A.; Tully, D.C.; Backes, B.J.; Harris, J.L.
Biochemical characterization of prostasin, a channel activating protease (2004), Biochem. Biophys. Res. Commun., 324, 953-963.

Search for more literature for 3.4.21.B6

Activating Compound

Activating Compound	Comment	Organism	Structure
CHAPS	increases enzyme activity up to 4fold with increasing concentration	Homo sapiens
DMSO	increases enzyme activity up to 2fold with increasing concentration	Homo sapiens
additional information	NP-40, Triton X-100, 15% glycerol, up to 0.5 mM DTT and 0.25 mM beta-mercaptoethanol have no effect on enzyme activity	Homo sapiens
ZnCl2	with acetyl-PRLR-7-amino-3-carbamoylmethyl-4-methylcoumarin	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Sf9 cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Aprotinin	-	Homo sapiens
Brij 35	activity decreases with increasing detergent concentration	Homo sapiens
CaCl2	-	Homo sapiens
CoCl2	-	Homo sapiens
CuCl2	-	Homo sapiens
EDTA	reduces enzyme activity by 30%	Homo sapiens
LiCl	-	Homo sapiens
MgCl2	-	Homo sapiens
additional information	not inhibited by alpha1-antitrypsin, alpha1-antitrypsinPDX, alpha1-antichymotrypsin, and soybean trypsin inhibitor	Homo sapiens
NaCl	-	Homo sapiens
NaF	-	Homo sapiens
NaI	-	Homo sapiens
NiCl2	-	Homo sapiens
placental bikunin	-	Homo sapiens
Tween 20	activity decreases with increasing detergent concentration	Homo sapiens
ZnCl2	with acetyl-KHYR-7-amino-3-carbamoylmethyl-4-methylcoumarin	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0555	-	acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin	with ZnCL2	Homo sapiens
0.0786	-	acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	without ZnCL2	Homo sapiens
0.141	-	acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	with ZnCL2	Homo sapiens
0.193	-	acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	-	Homo sapiens
0.219	-	acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin	without ZnCL2	Homo sapiens
0.86	-	acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	SDS-PAGE, glycosylated enzyme	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-amido-4-carbamoylmethylcoumarin-KHYRSVAS-K(DNP)R + H2O	-	Homo sapiens	?	-	?
7-amido-4-carbamoylmethylcoumarin-KHYRSVAW-K(DNP)R + H2O	-	Homo sapiens	?	-	?
acetyl-DHYR-7-amido-4-carbamoylmethylcoumarin + H2O	suboptimal substrate	Homo sapiens	acetyl-DHYR + 7-amino-4-carbamoylmethylcoumarin	-	?
acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin + H2O	suboptimal substrate	Homo sapiens	acetyl-KDYR + 7-amino-4-carbamoylmethylcoumarin	-	?
acetyl-KHDR-7-amido-4-carbamoylmethylcoumarin + H2O	suboptimal substrate	Homo sapiens	acetyl-KHDR + 7-amino-4-carbamoylmethylcoumarin	-	?
acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin + H2O	-	Homo sapiens	acetyl-KHYR + 7-amino-4-carbamoylmethylcoumarin	-	?
acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin + H2O	optimal substrate	Homo sapiens	acetyl-KHYR + 7-amino-4-carbamoylmethylcoumarin	-	?
acetyl-KHYR-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	acetyl-KHYR + 7-amino-4-methylcoumarin	-	?
acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin + H2O	suboptimal substrate	Homo sapiens	acetyl-PRLR + 7-amino-4-carbamoylmethylcoumarin	-	?
additional information	7-amido-4-carbamoylmethylcoumarin-KHYRIVAS-K(DNP)R completely inactive	Homo sapiens	?	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.083	-	acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin	-	Homo sapiens
0.381	-	acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	with ZnCL2	Homo sapiens
0.601	-	acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	without ZnCL2	Homo sapiens
1.97	-	acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	-	Homo sapiens
2.38	-	acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin	without ZnCL2	Homo sapiens
6.08	-	acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	without ZnCL2	Homo sapiens
6.53	-	acetyl-KDYR-7-amido-4-carbamoylmethylcoumarin	with ZnCL2	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000014	-	Aprotinin	-	Homo sapiens
0.000005	-	placental bikunin	-	Homo sapiens
0.0021	-	CuCl2	with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
0.0048	-	ZnCl2	with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
0.01	-	NiCl2	with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
0.047	-	CoCl2	with acetyl-PRLR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
0.57	-	CoCl2	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
1.15	-	CaCl2	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
1.42	-	MgCl2	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
16.4	-	NaI	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
21.3	-	LiCl	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
22.4	-	NaCl	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens
26.3	-	NaF	with acetyl-KHYR-7-amido-4-carbamoylmethylcoumarin	Homo sapiens

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Release 2023.1 (January 2023)