Cloned (Comment) | Organism |
---|---|
recombinant expression of enzymatically active enzyme in RBL-2H3 granules | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S202A | inactive mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DFF45 protein + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P20718 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | granzyme H is a serine protease, initially produced as a zymogen that requires the activity of granule-resident cathepsin C to become active. Cell death in cytotoxic potential of the enzyme with replication-deficient adenovirus 5 in target cells, i.e. K562 cells, Jurkat cells, and EBV-transformed B cell line RPMI-8866,the enzyme is less effective against tumor cells. Enzyme GrH induces cytochrome C loss from the mitochondrial intermembrane space. Similar to cytochrome C, SMAC/DIABLO is also observed to undergo translocation from the mitochondria to the cytosol after treatment with the enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
isolation of enzymatically active recombinant wild-type enzyme and of inactive mutant S202A from RBL-2H3 granules | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
JURKAT cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
natural killer cell | - |
Homo sapiens | - |
serum | - |
Homo sapiens | - |
T-lymphocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DFF45 protein + H2O | - |
Homo sapiens | ? | - |
? | |
DFF45 protein + H2O | GrH directly processes DFF45/ICAD to cleavage products of 30 kDa and 15 kDa | Homo sapiens | ? | - |
? | |
additional information | granzyme H does not efficiently process Bid | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Granzyme H | - |
Homo sapiens |
GrH | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | Bid is not required for enzyme-mediated cell death | Homo sapiens |
physiological function | the enzyme induces cell death primarily via a Bcl-2-sensitive mitochondrial cell death pathway that does not require direct Bid activation, overview. Neither the apoptosome nor caspase-3 is essential to the induction of GrH-mediated cell death. The enzyme might have pro-apoptotic activity that might be distinct from that of granzyme B and FasL, which can be relevant when Fas/FasL or granzyme B activity or death pathways are impaired. Direct processing of DFF45 by the enzyme may be leading to DNA damage. The enzyme inhibits caspase-3 activation | Homo sapiens |