Literature summary for 3.4.21.B57 extracted from

Uehara, R.; Tanaka, S.; Takano, K.; Koga, Y.; Kanaya, S.
Requirement of insertion sequence IS1 for thermal adaptation of Pro-Tk-subtilisin from hyperthermophilic archaeon (2012), Extremophiles, 16, 841-851.

Search for more literature for 3.4.21.B57

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wid-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Thermococcus kodakarensis

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of IS1-deletion mutants of S324A and S324C enzyme variants	Thermococcus kodakarensis
S324A	site-directed mutagenesis, structure comparison of the mutant pro-enzyme with the wild-type pro-enzyme	Thermococcus kodakarensis
S324C	site-directed mutagenesis, structure comparison of the mutant pro-enzyme with the wild-type pro-enzyme	Thermococcus kodakarensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted into the external medium, probably as inactive pro-enzyme, with the assistance of the signal sequence	Thermococcus kodakarensis	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Thermococcus kodakarensis	the propeptide is effectively degraded by the mature enzyme only at high temperatures, because it is too stable to be degraded at moderate temperatures	?	-	?
pro-Tk-subtilisin + H2O	Thermococcus kodakarensis	autoactivation	Tk-subtilisin + propeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	P58502	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	prepro-Tk-subtilisin (Prepro-TKS), which consists of the signal sequence [Met (-24)-Ala(-1)], propeptide (Gly1-Leu69), and mature domain (Tk-subtilisin, Gly70-Gly398). Tk-subtilisin matures from Pro-Tk-subtilisin upon autoprocessing and degradation of propeptide. The pro-enzyme form contains the insertion sequence, IS1, at the N-terminus of the mature domain which is required not only for hyperstabilization of Pro-Tk-subtilisin but also for its rapid maturation, Most part of IS1 (Gly70-Gly78) is autocatalytically removed when Pro-TKS matures to Tk-subtilisin, structure and mechanism, overview	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the propeptide is effectively degraded by the mature enzyme only at high temperatures, because it is too stable to be degraded at moderate temperatures	Thermococcus kodakarensis	?	-	?
pro-Tk-subtilisin + H2O	autoactivation	Thermococcus kodakarensis	Tk-subtilisin + propeptide	-	?

Synonyms

Synonyms	Comment	Organism
Tk-subtilisin	-	Thermococcus kodakarensis
TKS	-	Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9.5	-	assay at	Thermococcus kodakarensis

General Information

General Information	Comment	Organism
additional information	prepro-Tk-subtilisin (Prepro-TKS), which consists of the signal sequence [Met (-24)-Ala(-1)], propeptide (Tkpro, Gly1-Leu69), and mature domain (Tk-subtilisin, Gly70-Gly398). the pro-enzyme form contains the insertion sequence, IS1, at the N-terminus of the mature domain which is required not only for hyperstabilization of Pro-TKS but also for its rapid maturation	Thermococcus kodakarensis

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Release 2023.1 (January 2023)