Application | Comment | Organism |
---|---|---|
medicine | the abnormal prion protein (scrapie-associated prion protein, PrP(Sc)) is considered to be included in the group of infectious agents of transmissible spongiform encephalopathies. Since PrP(Sc) is highly resistant to normal sterilization procedures, the decontamination of PrP(Sc) is a significant public health issue. The hyperthermostable protease, Tk-subtilisin, can be an effective agent for medical decontamination of of PrP(Sc). Rather small amounts of Tk-subtilisin (0.3 U) are required to degrade PrP(Sc) at 100 °C and pH 8.0. In addition, Tk-subtilisin is observed to degrade PrP(Sc) in the presence of sodium dodecyl sulfate or other industrial surfactants. Basic information for the practical use of the proteolytic enzyme for PrP(Sc) degradation is provided | Thermococcus kodakarensis |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Thermococcus kodakarensis |
General Stability | Organism |
---|---|
the activity is retained or even enhanced in the presence of nonionic, cationic (except in the presence of SANIZOL C), and amphoteric surfactants at both 0.1 and 1% (w/v) | Thermococcus kodakarensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DFP | - |
Thermococcus kodakarensis | |
additional information | the activity is retained or even enhanced in the presence of nonionic, cationic (except in the presence of SANIZOL C), and amphoteric surfactants at both 0.1 and 1% (w/v) | Thermococcus kodakarensis |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | Tk-subtilisin retains its specific activity in 6 M guanidine HCl after 60 min incubation at room temperature | Thermococcus kodakarensis |
SDS | Tk-subtilisin shows no significant reduction of specific activity after 60 min incubation at room temperature with 5% SDS | Thermococcus kodakarensis |
urea | Tk-subtilisin retains its specific activity in 8 M urea after 60 min incubation at room temperature | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | P58502 | sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106) | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
abnormal prion protein PrP(Sc) + H2O | the abnormal prion protein (scrapie-associated prion protein, PrP(Sc)) is considered to be included in the group of infectious agents of transmissible spongiform encephalopathies. Although PrP(Sc) is known to be resistant toward proteolytic enzymes, Tk-subtilisin is able to degrade PrP(Sc) under extreme conditions | Thermococcus kodakarensis | ? | - |
? | |
N-succinyl-AAPF-4-nitroanilide + H2O | - |
Thermococcus kodakarensis | N-succinyl-AAPF + 4-nitroaniline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Thermococcus kodakarensis |