Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli in a form with a putative prosequence in inclusion bodies, solubilized in the presence of 8 M urea, and refolded and converted to an active molecule. The enzyme is refolded in a form with a putative prosequence | Thermococcus kodakarensis |
General Stability | Organism |
---|---|
the enzyme is stabilized in the presence of the Ca2+ | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | highest activity in the presence of 5 mM CaCl2. The enzyme exhibits 70% and 80% of the maximal activity in the presence of 1 and 100 mM CaCl2, respectively | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Thermococcus kodakarensis |
43783 | - |
1 * 43783, calculation from sequence | Thermococcus kodakarensis |
44000 | - |
1 * 44000, SDS-PAGE | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | P58502 | sequence including singnal peptide (amino acid 1-24) and propeptide (amino acid 25-106) | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | broad substrate specificity, with a slight preference for aromatic or large nonpolar P1 substrate residues | Thermococcus kodakarensis | ? | - |
? | |
N-succinyl-Ala-Ala-Pro-Leu-4-nitroanilide + H2O | 24.3% compared to the activity with N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide | Thermococcus kodakarensis | N-succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline | - |
? | |
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Thermococcus kodakarensis | N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 44000, SDS-PAGE | Thermococcus kodakarensis |
monomer | 1 * 43783, calculation from sequence | Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | the enzyme exhibits 10% to 20% of the maximal activity at 40°C or 90°C | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half life: more than 60 min, in the presence of 50 mM CaCl2 | Thermococcus kodakarensis |
90 | - |
half life: 20 min, in the presence of 50 mM CaCl2 | Thermococcus kodakarensis |
100 | - |
half life: 7 min, in the presence of 50 mM CaCl2 | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Thermococcus kodakarensis |