General Stability | Organism |
---|---|
the enzyme is stable for 60 min in buffer containing 1 M NaCl at 50°C, it loses 96% of its activity after 60 min in 1 mM NaCl at 95°C | Saccharolobus solfataricus |
the enzyme loses 54% of its activity after 60 min in buffer containing 2 mM dithiothreitol at 95°C | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymostatin | 0.3 mg/ml, 94% inhibition | Saccharolobus solfataricus | |
iodoacetate | 1 mM, 10% inhibition | Saccharolobus solfataricus | |
N-alpha-p-tosyl-L-lysine chloromethyl ketone | 0.3 mM, 6% inhibition | Saccharolobus solfataricus | |
p-chloromercuribenzoate | 0.6 mM, 6% inhibition | Saccharolobus solfataricus | |
phenylmethanesulfonyl fluoride | 5 mM, 98% inhibition | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
benzyloxycarbonyl-Tyr-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
0.34 | - |
benzyloxycarbonyl-Leu-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
0.72 | - |
Suc-Gly-Gly-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
0.85 | - |
Suc-Ala-Ala-Pro-Phe 4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
1.16 | - |
Suc-Ala-Ala-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
1.34 | - |
Suc-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Saccharolobus solfataricus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation effect up to 2 mM | Saccharolobus solfataricus | |
Mg2+ | no effect by Mg2+, Ca2+ and Zn2+ over the concentration range 0.2-20 mM | Saccharolobus solfataricus | |
Mn2+ | significantly stimulates, mixed type non-essential activator | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Saccharolobus solfataricus |
118000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 50% acetonitrile, 91% inhibition after 30 min at 50°C | Saccharolobus solfataricus |
Ethanol | enzyme can withstand 50% ethanol at 50°C, 98% loss of activity after 30 min at 95°C. 45% loss of activity after 30 min, in 99% ethanol | Saccharolobus solfataricus |
Glycerol | 20% glycerol, 35% inhibition after 30 min at 95°C | Saccharolobus solfataricus |
Methanol | 50% methanol, 66% loss of activity after 30 min at 50°C, 98% loss of activity after 30 min, at 95°C | Saccharolobus solfataricus |
tetrahydrofuran | 50% tetrahydrofuran, 77% inhibition after 30 min at 50°C, 96% inhibition after 30 min at 95°C | Saccharolobus solfataricus |
urea | enzyme can withstand 6 M urea at 50°C, 97% loss of activity after 30 min at 95°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.9 | - |
pH 7.0, 80°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Leu-4-nitroanilide + H2O | 1% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide | Saccharolobus solfataricus | benzyloxycarbonyl-Ala-Ala-Leu + 4-nitroaniline | - |
? | |
benzyloxycarbonyl-Leu-4-nitroanilide + H2O | 36% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide | Saccharolobus solfataricus | benzyloxycarbonyl-Leu + 4-nitroaniline | - |
? | |
benzyloxycarbonyl-Tyr-4-nitroanilide + H2O | - |
Saccharolobus solfataricus | benzyloxycarbonyl-Tyr + 4-nitroaniline | - |
? | |
additional information | narrow substrate specificity. No activity with: aminoacyl-4-nitroanilides or benzyloxycarbonyl-amino acids, oxidized insulin chain A and B, bovine pancreas ribonuclease A, bovine serum albumin, ovalbumin. No activity with benzyloxycarbonyl-Ile-4-nitroanilide | Saccharolobus solfataricus | ? | - |
? | |
Phe-Ser-Lys-Phe-Ile-Ser-Phe-Asp + H2O | no cleavage between Phe4 and Ile5 | Saccharolobus solfataricus | Phe-Ser-Lys-Phe-Ile + Ser-Phe-Asp | - |
? | |
Suc-Ala-Ala-Phe-4-nitroanilide + H2O | 3% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide | Saccharolobus solfataricus | Suc-Ala-Ala-Phe + 4-nitroaniline | - |
? | |
Suc-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | 2% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide | Saccharolobus solfataricus | Suc-Ala-Ala-Pro-Phe + 4-nitroaniline | - |
? | |
Suc-Gly-Gly-Phe-4-nitroanilide + H2O | 6.5% of the activity with benzyloxycarbonyl-Tyr-4-nitroanilide | Saccharolobus solfataricus | Suc-Gly-Gly-Phe + 4-nitroaniline | - |
? | |
Suc-Phe-4-nitroanilide + H2O | 18.5% of the activity with benzyloxycarbonyl-Tyr-p-nitroanilide | Saccharolobus solfataricus | Suc-Phe + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme can withstand 6 M urea | Saccharolobus solfataricus |
90 | - |
completly stable up to | Saccharolobus solfataricus |
92 | - |
first-order thermal inactivation, half-life: 342 min | Saccharolobus solfataricus |
101 | - |
first-order thermal inactivation, half-life: 7 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Suc-Ala-Ala-Pro-Phe 4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
2.2 | - |
Suc-Ala-Ala-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
3.3 | - |
Suc-Gly-Gly-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
12.1 | - |
benzyloxycarbonyl-Leu-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
15.6 | - |
Suc-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
23 | - |
benzyloxycarbonyl-Tyr-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Saccharolobus solfataricus |
7.5 | - |
in absence of divalent cations | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
5.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
Suc-Ala-Ala-Pro-Phe 4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
1.9 | - |
Suc-Ala-Ala-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
4.6 | - |
Suc-Gly-Gly-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
10.7 | - |
Suc-Phe-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
35 | - |
benzyloxycarbonyl-Leu-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus | |
210 | - |
benzyloxycarbonyl-Tyr-4-nitroanilide | pH 7.0, 80°C | Saccharolobus solfataricus |