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Literature summary for 3.4.21.B49 extracted from

  • Campo, N.; Rudner, D.Z.
    A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis (2006), Mol. Cell, 23, 25-35.
    View publication on PubMed
Search for more literature for 3.4.21.B49

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli BL21(DE3) pLys Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
S309A unable to cleave SpoIVFA Bacillus subtilis

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information intermembrane space between the forespore and the mother cell membrane Bacillus subtilis
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-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
SpoIVFA + H2O Bacillus subtilis enzyme can substitute the function of SpoIVB, involved in sigmaK processing fragements of SpoIVFA
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
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-
-
Bacillus subtilis PY79
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
His tagged extracellular domain of SpoIVFA + H2O two cleavage sites identified, cleavage sites different from those of SpoIVB Bacillus subtilis ?
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 ?
additional information cleavage of BofA not detectable in an in vitro assay Bacillus subtilis ?
-
 ?
SpoIVFA + H2O enzyme can substitute the function of SpoIVB, involved in sigmaK processing Bacillus subtilis fragements of SpoIVFA
-
 ?

Synonyms

Synonyms Comment Organism
CtpB serine protease with a domain structure that resembles those of SpoIVB Bacillus subtilis