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Literature summary for 3.4.21.B12 extracted from

  • Lisle, J.E.; Mertens-Walker, I.; Stephens, C.R.; Stansfield, S.H.; Clements, J.A.; Herington, A.C.; Stephenson, S.A.
    Murine, but not human, ephrin-B2 can be efficiently cleaved by the serine protease kallikrein-4: implications for xenograft models of human prostate cancer (2015), Exp. Cell Res., 333, 136-146.
    View publication on PubMed
Search for more literature for 3.4.21.B12

Cloned(Commentary)

Cloned (Comment) Organism
gene KLK4, coupling of the coding sequence of mature enzyme to the pro-domain of prostate specific antigen at the 5' end, and V5 and poly-histidine encoding sequences at the 3' end in plasmid pIBm recombinant expression as inactive zymogen in Spodoptera frugiperda insect cell line Sf9 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme performs proteolytic autoactivation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9YSK2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme performs proteolytic autoactivation Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
human ephrin-B2 + H2O low activity with recombinant human enzyme and human substrate Homo sapiens ?
-
 ?
additional information the enzyme performs proteolytic autoactivation Homo sapiens ?
-
 ?
additional information position of predicted enzyme cleavage sites within the sequence and the folded protein, structure, overview Homo sapiens ?
-
 ?
murine ephrin-B2 + H2O the substrate harbors N-glycosylation sites its extracellular domain sequence, R27-R178, good activity with recombinant human enzyme and murine substrate, the primary enzyme cleavage site in murine ephrin-B2 is verified as located between extracellular domain residues Arg178 and N179 Homo sapiens ? cleavage fragments, overview ?

Synonyms

Synonyms Comment Organism
kallikrein-4
-
 Homo sapiens
KLK4
-
 Homo sapiens
prostate cancer serine protease
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme substrate murine ephrin-B2 is glycosylated and this post-translational modification may regulate the enzyme cleavage activity Homo sapiens