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Literature summary for 3.4.21.B12 extracted from
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins (2006), Biol. Chem., 387, 749-759.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.049 | - |
VPR-7-amido-4-methylcoumarin | pH 8.0, 37°C | Homo sapiens |  |
| 0.052 | - |
VLK-7-amido-4-methylcoumarin | pH 8.0, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 30000, glycosylated form, x * 25000, deglycosylated form, SDS-PAGE | Homo sapiens |
| 30000 | - |
x * 30000, glycosylated form, x * 25000, deglycosylated form, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme is involved in tissue remodeling and cancer metastasis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
expressed in Pichia pastoris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| collagen I + H2O | limited degradation | Homo sapiens | ? | - |
? | |
| Collagen IV + H2O | limited degradation | Homo sapiens | ? | - |
? | |
| EKK-7-amido-4-methylcoumarin + H2O | 9% of the activity with VPR-7-amido-4-methylcoumarin | Homo sapiens | EKK + 7-amino-4-methylcoumarin | - |
? | |
| fibrinogen alpha-chain + H2O | - |
Homo sapiens | ? | - |
? | |
| GPR-7-amido-4-methylcoumarin + H2O | 20% of the activity with VPR-7-amido-4-methylcoumarin | Homo sapiens | GPR + 7-amino-4-methylcoumarin | - |
? | |
| additional information | enzyme is involved in tissue remodeling and cancer metastasis | Homo sapiens | ? | - |
? | |
| additional information | enzyme cleaves more efficiently after Arg compared to Lys at the P1 position and exhibits modest specificity for amino acids at P2 and P3 positions | Homo sapiens | ? | - |
? | |
| QAR-7-amido-4-methylcoumarin + H2O | 38% of the activity with VPR-7-amido-4-methylcoumarin | Homo sapiens | QAR + 7-amino-4-methylcoumarin | - |
? | |
| VLK-7-amido-4-methylcoumarin + H2O | 60% of the activity with VPR-7-amido-4-methylcoumarin | Homo sapiens | VLK + 7-amino-4-methylcoumarin | - |
? | |
| VPR-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | VPR + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, glycosylated form, x * 25000, deglycosylated form, SDS-PAGE | Homo sapiens |
| More | enzyme forms complexes with alpha1-antitrypsin, alpha2-antiplasmin and alpha2-macroglobulin | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0078 | - |
VLK-7-amido-4-methylcoumarin | pH 8.0, 37°C | Homo sapiens | |
| 0.033 | - |
VPR-7-amido-4-methylcoumarin | pH 8.0, 37°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
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Release 2023.1 (January 2023)
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