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Literature summary for 3.4.21.B12 extracted from

  • Ryu, O.; Hu, J.C.; Yamakoshi, Y.; Villemain, J.L.; Cao, X.; Zhang, C.; Bartlett, J.D.; Simmer, J.P.
    Porcine kallikrein-4 activation, glycosylation, activity, and expression in prokaryotic and eukaryotic hosts (2002), Eur. J. Oral Sci., 110, 358-365.
    View publication on PubMed
Search for more literature for 3.4.21.B12

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation of recombinant zymogen by native KLK4 enzyme in vitro Sus scrofa
recombinant pig enamelysin can activate the recombinant zymogen in vitro Sus scrofa
Thermolysin can activate the recombinant zymogen in vitro Sus scrofa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain AD 494 (DE3) with and without the pro-peptide Sus scrofa
expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus Sus scrofa
expression of inactive zymogen in human 293 kidney cells Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sus scrofa enzyme participates in the degradation of enamel proteins in vivo ?
-
 ?
polypeptide + H2O Sus scrofa
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa Q9XSN6 gene KLK4
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2 N-glycosylated forms of different MW: 34 kDa and 37 kDa Sus scrofa
glycoprotein deglycosylation abolishes enzyme activity Sus scrofa
proteolytic modification no activation of recombinant zymogen by native KLK4 enzyme in vitro Sus scrofa
proteolytic modification recombinant zymogen can be activated in vitro by thermolysin and by recombinant pig enamelysin Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
native enzyme from developing teeth Sus scrofa
recombinant enzyme from 293 kidney cells Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
proteolysis of polypeptides enzyme is a serine protease Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
enamel transition-stage developing matrix Sus scrofa
-
tooth
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amelogenin + H2O recombinant substrate from pig expressed in Escherichia coli strain XL-1 blue as His-tagged protein Sus scrofa amelogenin fragments product identification by N-terminal sequencing and mass spectrometry ?
casein + H2O zymograph assay Sus scrofa ?
-
 ?
Gelatin + H2O zymograph assay Sus scrofa ?
-
 ?
additional information recombinant zymogen can be activated by thermolysin Sus scrofa ?
-
 ?
additional information enzyme participates in the degradation of enamel proteins in vivo Sus scrofa ?
-
 ?
polypeptide + H2O
-
 Sus scrofa peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 34000-37000, glycosylated enzyme, 2 glycosylation forms, SDS-PAGE Sus scrofa
? x * 28000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Sus scrofa