BRENDA - Enzyme Database
show all sequences of 3.4.21.99

Evidence for the participation of two sperm proteases, spermosin and acrosin, in fertilization of the ascidian, Halocynthia roretzi: inhibitory effects of leupeptin analogs on enzyme activities and fertilization

Sawada, H.; Yokosawa, H.; Someno, T.; Saino, T.; Ishii, S.; Dev. Biol. 105, 246-249 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5-pyrrolidone-2-carboxyl-Leu-DL-arginal
0.01 mM, 25% inhibition
Halocynthia roretzi
dansyl-Leu-DL-arginal
0.01 mM, 24% inhibition
Halocynthia roretzi
N-acetyl-Leu-Leu-arginal
i.e. leupeptin, 0.01 mM, 55% inhibition
Halocynthia roretzi
N-benzoyl-Leu-arginal
0.01 mM, 15% inhibition
Halocynthia roretzi
N-benzyloxycarbonyl-Phe-Leu-arginal
0.01 mM, 93% inhibition
Halocynthia roretzi
N-benzyloxycarbonyl-Val-Pro-arginal
0.01 mM, 99% inhibition
Halocynthia roretzi
o-nitrophenyl-Leu-arginal
0.01 mM, 52% inhibition
Halocynthia roretzi
p-nitrobenzyl-Leu-arginal
0.01 mM, 26% inhibition
Halocynthia roretzi
Pro-Leu-arginal
0.01 mM, 27% inhibition
Halocynthia roretzi
Organism
Organism
UniProt
Commentary
Textmining
Halocynthia roretzi
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spermatozoon
-
Halocynthia roretzi
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
137255
Halocynthia roretzi
tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
137255
Halocynthia roretzi
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-pyrrolidone-2-carboxyl-Leu-DL-arginal
0.01 mM, 25% inhibition
Halocynthia roretzi
dansyl-Leu-DL-arginal
0.01 mM, 24% inhibition
Halocynthia roretzi
N-acetyl-Leu-Leu-arginal
i.e. leupeptin, 0.01 mM, 55% inhibition
Halocynthia roretzi
N-benzoyl-Leu-arginal
0.01 mM, 15% inhibition
Halocynthia roretzi
N-benzyloxycarbonyl-Phe-Leu-arginal
0.01 mM, 93% inhibition
Halocynthia roretzi
N-benzyloxycarbonyl-Val-Pro-arginal
0.01 mM, 99% inhibition
Halocynthia roretzi
o-nitrophenyl-Leu-arginal
0.01 mM, 52% inhibition
Halocynthia roretzi
p-nitrobenzyl-Leu-arginal
0.01 mM, 26% inhibition
Halocynthia roretzi
Pro-Leu-arginal
0.01 mM, 27% inhibition
Halocynthia roretzi
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spermatozoon
-
Halocynthia roretzi
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
137255
Halocynthia roretzi
tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
137255
Halocynthia roretzi
?
Other publictions for EC 3.4.21.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665085
Lindsay
-
Spermosin ...
Halocynthia roretzi
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1567-1569
2004
-
-
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-
-
1
13
1
-
1
2
1
-
1
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1
1
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1
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3
-
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1
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1
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1
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13
-
1
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1
2
1
-
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1
1
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1
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-
3
-
-
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-
1
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-
1
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-
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-
-
-
651146
Kodama
Spermosin, a trypsin-like prot ...
Halocynthia roretzi
Eur. J. Biochem.
269
657-663
2002
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-
1
-
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2
1
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3
-
1
1
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3
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2
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1
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2
1
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1
1
-
3
-
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2
-
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-
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-
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-
-
-
137256
Sawada
Localization, expression, and ...
Halocynthia roretzi
Biochem. Biophys. Res. Commun.
222
499-504
1996
-
-
-
-
-
-
-
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1
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1
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1
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2
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1
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1
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2
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137257
Sawada
Substrate specificity of ascid ...
Halocynthia roretzi
Mol. Reprod. Dev.
45
240-243
1996
-
-
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3
-
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2
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1
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2
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1
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137258
Sawada
-
Timing of action of sperm prot ...
Halocynthia roretzi
Experientia
42
74-75
1986
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-
-
-
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1
-
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1
-
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1
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1
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1
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1
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1
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137254
Sawada
Purification and characterizat ...
Halocynthia roretzi
J. Biol. Chem.
259
2900-2904
1984
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3
12
1
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2
1
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8
-
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1
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4
1
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1
1
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1
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3
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12
-
1
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2
1
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1
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4
1
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1
1
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1
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-
137255
Sawada
Evidence for the participation ...
Halocynthia roretzi
Dev. Biol.
105
246-249
1984
-
-
-
-
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-
9
-
-
-
-
-
-
5
-
-
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-
-
3
-
-
1
-
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9
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3
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1
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