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Literature summary for 3.4.21.99 extracted from
- Purification and characterization of two types of trypsin-like enzymes from sperm of the ascidian (Prochordata) Halocynthia roretzi. Evidence for the presence of spermosin, a novel acrosin-like enzyme (1984), J. Biol. Chem., 259, 2900-2904.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin stabilizes | Halocynthia roretzi |
| non-ionic detergents such as Brij 35, Triton X-100, Tween 80 at concentrations between 0.005% and 0.1% stabilize | Halocynthia roretzi |
| stable in presence of contaminating proteins, very labile in their absence | Halocynthia roretzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antipain | - |
Halocynthia roretzi |  |
| benzamidine | - |
Halocynthia roretzi | |
| diisopropyl fluorophosphate | - |
Halocynthia roretzi | |
| EGTA | - |
Halocynthia roretzi | |
| Gly-Val-Arg-CH2Cl | - |
Halocynthia roretzi | |
| HgCl2 | - |
Halocynthia roretzi | |
| N-acetyl-Leu-Leu-arginal | - |
Halocynthia roretzi | |
| ovomucoid | - |
Halocynthia roretzi | |
| p-aminobenzamidine | - |
Halocynthia roretzi | |
| phenylmethanesulfonyl fluoride | - |
Halocynthia roretzi | |
| Soybean trypsin inhibitor | - |
Halocynthia roretzi | |
| ZnCl2 | - |
Halocynthia roretzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.145 | - |
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide | - |
Halocynthia roretzi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | maximum activity with 1-5 mM Ca2+, the presence of Ca2+ is not indispensable | Halocynthia roretzi | |
| additional information | not strongly influenced by changing salt concentrations except Ca2+ | Halocynthia roretzi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration, SDS-PAGE | Halocynthia roretzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halocynthia roretzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Halocynthia roretzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spermatozoon | - |
Halocynthia roretzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
- |
Halocynthia roretzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O | - |
Halocynthia roretzi | tert-butyloxycarbonyl-Val-Pro + Arg-4-methylcoumarin-7-amide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, SDS-PAGE | Halocynthia roretzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Halocynthia roretzi |
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Release 2023.1 (January 2023)
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