BRENDA - Enzyme Database
show all sequences of 3.4.21.99

Purification and characterization of two types of trypsin-like enzymes from sperm of the ascidian (Prochordata) Halocynthia roretzi. Evidence for the presence of spermosin, a novel acrosin-like enzyme

Sawada, H.; Yokosawa, H.; Ishii, S.; J. Biol. Chem. 259, 2900-2904 (1984)

Data extracted from this reference:

General Stability
General Stability
Organism
bovine serum albumin stabilizes
Halocynthia roretzi
non-ionic detergents such as Brij 35, Triton X-100, Tween 80 at concentrations between 0.005% and 0.1% stabilize
Halocynthia roretzi
stable in presence of contaminating proteins, very labile in their absence
Halocynthia roretzi
Inhibitors
Inhibitors
Commentary
Organism
Structure
antipain
-
Halocynthia roretzi
benzamidine
-
Halocynthia roretzi
diisopropyl fluorophosphate
-
Halocynthia roretzi
EGTA
-
Halocynthia roretzi
Gly-Val-Arg-CH2Cl
-
Halocynthia roretzi
HgCl2
-
Halocynthia roretzi
N-acetyl-Leu-Leu-arginal
-
Halocynthia roretzi
ovomucoid
-
Halocynthia roretzi
p-aminobenzamidine
-
Halocynthia roretzi
phenylmethanesulfonyl fluoride
-
Halocynthia roretzi
Soybean trypsin inhibitor
-
Halocynthia roretzi
ZnCl2
-
Halocynthia roretzi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.145
-
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide
-
Halocynthia roretzi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
maximum activity with 1-5 mM Ca2+, the presence of Ca2+ is not indispensable
Halocynthia roretzi
additional information
not strongly influenced by changing salt concentrations except Ca2+
Halocynthia roretzi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
gel filtration, SDS-PAGE
Halocynthia roretzi
Organism
Organism
UniProt
Commentary
Textmining
Halocynthia roretzi
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Halocynthia roretzi
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spermatozoon
-
Halocynthia roretzi
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2
-
-
Halocynthia roretzi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
137254
Halocynthia roretzi
tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
137254
Halocynthia roretzi
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 27000, SDS-PAGE
Halocynthia roretzi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Halocynthia roretzi
General Stability (protein specific)
General Stability
Organism
bovine serum albumin stabilizes
Halocynthia roretzi
non-ionic detergents such as Brij 35, Triton X-100, Tween 80 at concentrations between 0.005% and 0.1% stabilize
Halocynthia roretzi
stable in presence of contaminating proteins, very labile in their absence
Halocynthia roretzi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
antipain
-
Halocynthia roretzi
benzamidine
-
Halocynthia roretzi
diisopropyl fluorophosphate
-
Halocynthia roretzi
EGTA
-
Halocynthia roretzi
Gly-Val-Arg-CH2Cl
-
Halocynthia roretzi
HgCl2
-
Halocynthia roretzi
N-acetyl-Leu-Leu-arginal
-
Halocynthia roretzi
ovomucoid
-
Halocynthia roretzi
p-aminobenzamidine
-
Halocynthia roretzi
phenylmethanesulfonyl fluoride
-
Halocynthia roretzi
Soybean trypsin inhibitor
-
Halocynthia roretzi
ZnCl2
-
Halocynthia roretzi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.145
-
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide
-
Halocynthia roretzi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
maximum activity with 1-5 mM Ca2+, the presence of Ca2+ is not indispensable
Halocynthia roretzi
additional information
not strongly influenced by changing salt concentrations except Ca2+
Halocynthia roretzi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
27000
-
gel filtration, SDS-PAGE
Halocynthia roretzi
Purification (Commentary) (protein specific)
Commentary
Organism
-
Halocynthia roretzi
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spermatozoon
-
Halocynthia roretzi
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2
-
-
Halocynthia roretzi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
137254
Halocynthia roretzi
tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
-
137254
Halocynthia roretzi
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 27000, SDS-PAGE
Halocynthia roretzi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
9
-
Halocynthia roretzi
Other publictions for EC 3.4.21.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665085
Lindsay
-
Spermosin ...
Halocynthia roretzi
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1567-1569
2004
-
-
-
-
-
1
13
1
-
1
2
1
-
1
-
1
1
-
-
1
-
-
3
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
13
-
1
-
1
2
1
-
-
1
1
-
1
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
651146
Kodama
Spermosin, a trypsin-like prot ...
Halocynthia roretzi
Eur. J. Biochem.
269
657-663
2002
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
1
1
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
-
-
1
1
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137256
Sawada
Localization, expression, and ...
Halocynthia roretzi
Biochem. Biophys. Res. Commun.
222
499-504
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137257
Sawada
Substrate specificity of ascid ...
Halocynthia roretzi
Mol. Reprod. Dev.
45
240-243
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137258
Sawada
-
Timing of action of sperm prot ...
Halocynthia roretzi
Experientia
42
74-75
1986
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137254
Sawada
Purification and characterizat ...
Halocynthia roretzi
J. Biol. Chem.
259
2900-2904
1984
-
-
-
-
-
3
12
1
-
2
1
-
-
8
-
-
1
-
-
4
1
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
12
-
1
-
2
1
-
-
-
-
1
-
4
1
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
137255
Sawada
Evidence for the participation ...
Halocynthia roretzi
Dev. Biol.
105
246-249
1984
-
-
-
-
-
-
9
-
-
-
-
-
-
5
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-