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Literature summary for 3.4.21.98 extracted from

  • Steinkuhler, C.; Urbani, A.; Tomei, L.; Biasiol., G.; Sardana, M.; Bianchi, E.; Pessi, A.; de Francesco, R.
    Activity of purified hepatitis C virus protease NS3 on peptide substrates (1996), J. Virol., 70, 6694-6700.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.98

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
 9-fluorenylmethyloxycarbonyl-YQEFDEMEECASHLPYIEQG
-
 Hepacivirus C
0.095
-
 Ac-YQEFDEMEECASHLP
-
 Hepacivirus C
0.11
-
 Ac-YQEFDEMEECASH
-
 Hepacivirus C
0.12
-
 Ac-YQEFDEMEECASHL
-
 Hepacivirus C
0.15
-
 Ac-YQEFDEMEECAS
-
 Hepacivirus C
3.1
-
 Ac-EMEECASHLP
-
 Hepacivirus C

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18810
-
 electrospray mass spectrometry Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9-fluorenylmethyloxycarbonyl-YQEFDEMEECASHLPYIEQG + H2O
-
 Hepacivirus C 9-fluorenylmethyloxycarbonyl-YQEFDEMEEC + ASHLPYIEQG
-
 ?
Ac-EMEECASHLP + H2O
-
 Hepacivirus C Ac-EMEEC + ASHLP
-
 ?
Ac-YQEFDEMEECAS + H2O
-
 Hepacivirus C Ac-YQEFDEMEEC + AS
-
 ?
Ac-YQEFDEMEECASH + H2O
-
 Hepacivirus C Ac-YQEFDEMEEC + ASH
-
 ?
Ac-YQEFDEMEECASHL + H2O
-
 Hepacivirus C Ac-YQEFDEMEEC + ASHL
-
 ?
Ac-YQEFDEMEECASHLP + H2O
-
 Hepacivirus C Ac-YQEFDEMEEC + ASHLP
-
 ?
additional information decameric peptide spanning from P6-P4' required as a minimum Hepacivirus C ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15
-
 half-life 60 min Hepacivirus C
23
-
 half-life 30 min Hepacivirus C
30
-
 half-life 10 min Hepacivirus C

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00333
-
 Ac-YQEFDEMEECASH
-
 Hepacivirus C
0.005
-
 Ac-EMEECASHLP
-
 Hepacivirus C
0.005
-
 Ac-YQEFDEMEECAS
-
 Hepacivirus C
0.00667
-
 Ac-YQEFDEMEECASHL
-
 Hepacivirus C
0.00667
-
 Ac-YQEFDEMEECASHLP
-
 Hepacivirus C
0.00833
-
 9-fluorenylmethyloxycarbonyl-YQEFDEMEECASHLPYIEQG
-
 Hepacivirus C

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Hepacivirus C

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 22°C, 3 h, 10% loss of activity Hepacivirus C
8.5
-
 22°C, 3 h, 25% loss of activity Hepacivirus C