Literature summary for 3.4.21.98 extracted from

Wilkinson, T.C.I.; Bunyard, P.R.; Quirk, K; Wilkinson, C.S.
Characterisation of an HCV NS3/NS4A proteinase fusion protein expressed in E.coli using synthetic peptide substrates (1997), Biochem. Soc. Trans., 25, S624.

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Activating Compound

Activating Compound	Comment	Organism	Structure
DTT	required for maximal activity	Hepacivirus C
Triton X-100	required for maximal activity	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-(4-dimethylaminophenylazo)benzoyl-DEMEECASHE-5-(2-aminoethylamino)-1-sulfonic acid + H2O	-	Hepacivirus C	4-(4-dimethylaminophenylazo)benzoyl-DEMEEC + ASHE-5-(2-aminoethylamino)-1-sulfonic acid	-	?
succinyl-DEMEECASHPYPY-NH2 + H2O	-	Hepacivirus C	succinyl-DEMEEC + ASHPYPY-NH2	-	?
succinyl-EDVVPCSMYSTW-NH2 + H2O	-	Hepacivirus C	?	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Hepacivirus C

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Release 2023.1 (January 2023)