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Literature summary for 3.4.21.98 extracted from

  • Dimasi, N.; Pasquo, A.; Martin, F.; di Marco, S.; Steinkuhler, C.; Cortese, R.; Sollazzo, M.
    Engineering, characterization and phage display of hepatitis C virus NS3 protease and NS4A cofactor peptide as a single-chain protein (1998), Protein Eng., 11, 1257-1265.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Hepacivirus C

Protein Variants

Protein Variants Comment Organism
additional information engineering and phage display of hepatitis C virus NS3 protease and NS4A cofactor peptide as a single-chain protein Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
NaCl
-
Hepacivirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.087
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.088
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.102
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.403
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C
0.49
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C
0.502
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C
0.519
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant single-chain protease Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-DEEMECASHLPYK-NH2 + H2O
-
Hepacivirus C Ac-DEEMEC + ASHLPYK-NH2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00167
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.00183
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C
0.0233
-
Ac-DEEMECASHLPYK-NH2 wild-type NS3 protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.0253
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.0255
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT Hepacivirus C
0.0267
-
Ac-DEEMECASHLPYK-NH2 wild-type or engineered single-chain protease plus cofactor NS4A, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C
0.03
-
Ac-DEEMECASHLPYK-NH2 engineered single-chain protease, in 50 mM HEPES buffer, pH 7.5, 0.1% 1-O-n-octyl-beta-D-glucopyranoside, 15% glycerol, 30 mM DTT, 150 mM NaCl Hepacivirus C