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Literature summary for 3.4.21.98 extracted from

  • Sardana, V.V.; Blue, J.T.; Zugay-Murphy, J.; Sardana, M.K.; Kuo, L.C.
    An uniquely purified HCV NS3 protease and NS4A(21-34) peptide form a highly active serine protease complex in peptide hydrolysis (1999), Protein Expr. Purif., 16, 440-447.
    View publication on PubMed
Search for more literature for 3.4.21.98

Activating Compound

Activating Compound Comment Organism Structure
NS4A enhances the activity of NS3 protease in all cleavages via the formation of an NS3/NS4A Hepacivirus C
NS4A hydrophobic central core of NS4A corresponding to residues 21-34, kinetic value for a modified peptide modified with a KK residue at the C-terminus to enhance solubility, Km: 0.00076 mM, kcat: 9 per min Hepacivirus C

Cloned(Commentary)

Cloned (Comment) Organism
residues 1027-1207 of the NS3 protein, expressed in Escherichia coli Hepacivirus C

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00076
-
 7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) presence of inhibitor peptide 4Apep21-34KK Hepacivirus C
0.0028
-
 acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) presence of inhibitor peptide 4Apep21-34KK Hepacivirus C
0.0292
-
 7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3)
-
 Hepacivirus C
0.0436
-
 7-methoxycoumarin-4-acetyl-EDASTPCSGSN-(4-nitro)Phe presence of inhibitor peptide 4Apep21-34KK Hepacivirus C

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18867
-
 1 * 18867, NS3 protease domain residues from 1029-1206, mass spectrometry Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hepacivirus C

Storage Stability

Storage Stability Organism
-80°C, 25 mM phosphate buffer, pH 7.5, 10% glycerol, 5 mM DTT Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) + H2O
-
 Hepacivirus C 7-methoxycoumarin-4-acetyl-DEMEEC + ASHLPYK-(epsilon-NHCOCH3)
-
 ?
7-methoxycoumarin-4-acetyl-EDASTPCSGSN-(4-nitro)Phe + H2O
-
 Hepacivirus C 7-methoxycoumarin-4-acetyl-EDASTPC + SGSN-(4-nitro)Phe
-
 ?
acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) + H2O
-
 Hepacivirus C acetyl-DEMEEC + ASHLPYK-(epsilon-NHCOCH3)
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 18867, NS3 protease domain residues from 1029-1206, mass spectrometry Hepacivirus C

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3)
-
 Hepacivirus C
0.143
-
 acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) presence of inhibitor peptide 4Apep21-34KK Hepacivirus C
0.15
-
 7-methoxycoumarin-4-acetyl-DEMEECASHLPYK-(epsilon-NHCOCH3) presence of inhibitor peptide 4Apep21-34KK Hepacivirus C
0.227
-
 7-methoxycoumarin-4-acetyl-EDASTPCSGSN-(4-nitro)Phe presence of inhibitor peptide 4Apep21-34KK Hepacivirus C