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Literature summary for 3.4.21.98 extracted from
- A screening method for identifying disruptions in interferon signaling reveals HCV NS3/4a disrupts Stat-1 phosphorylation (2008), Antiviral Res., 77, 169-176.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | in order to develop an efficient screening method to identify viral proteins and their ability to block Jak-Stat signaling, the 2FTGH (human fibrosarcoma cells) cell assay system is used in combination with transient transfection of hepatitis C virus proteins: Using 1000 U/ml interferon and 30 mM 6-thioguanine to treat 2FTGH cells, it is established that transient protein expression in this cell system yields 39% and 0% cell survival for the positive (HPV E7) and negative controls (GFP expression) respectively. Transient expression of HCV Core-p7 results in 22% cell survival, consistent with previous reports, while expression of the HCV serine protease NS3/4a results in 54% cell survival. Furthermore, it is shown that NS3/4a inhibits phosphorylation of Stat-1 at the serine residue | Hepacivirus C |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hepacivirus C | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NS3/4a | - |
Hepacivirus C |
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