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Literature summary for 3.4.21.98 extracted from

  • Franco, S.; Clotet, B.; Martinez, M.A.
    A wide range of NS3/4A protease catalytic efficiencies in HCV-infected individuals (2008), Virus Res., 131, 260-270.
    View publication on PubMed

Application

Application Comment Organism
drug development genotype 1a, different catalytic efficiencies among NS3/4A proteases from different or similar hepatitis C virus genotypes. Because modulation of enzymatic activity of the protease is dependent on the interactions of a small number of NS4A amino acid residues, the design of small peptidomimetic protease inhibitors may be feasible Hepacivirus C
drug development genotype 1b, different catalytic efficiencies among NS3/4A proteases from different or similar hepatitis C virus genotypes. Because modulation of enzymatic activity of the protease is dependent on the interactions of a small number of NS4A amino acid residues, the design of small peptidomimetic protease inhibitors may be feasible Hepacivirus C
drug development genotype 3a, different catalytic efficiencies among NS3/4A proteases from different or similar hepatitis C virus genotypes. Because modulation of enzymatic activity of the protease is dependent on the interactions of a small number of NS4A amino acid residues, the design of small peptidomimetic protease inhibitors may be feasible Hepacivirus C
drug development genotype 4a, different catalytic efficiencies among NS3/4A proteases from different or similar hepatitis C virus genotypes. Because modulation of enzymatic activity of the protease is dependent on the interactions of a small number of NS4A amino acid residues, the design of small peptidomimetic protease inhibitors may be feasible Hepacivirus C
drug development genotype 4d, different catalytic efficiencies among NS3/4A proteases from different or similar hepatitis C virus genotypes. Because modulation of enzymatic activity of the protease is dependent on the interactions of a small number of NS4A amino acid residues, the design of small peptidomimetic protease inhibitors may be feasible Hepacivirus C

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli JM109 cells containig plasmid pcI.HCVcro that includes the NS5A/NS5B cleavage site transformed with plasmid pHCVNS32-181/421-34 protease. Expression in Escherichia coli XL-1 Blue cells Hepacivirus C
Escherichia coli JM109 cells containing plasmid pcI.HCVcro that includes the NS5A/NS5B cleavage site transformed with plasmid pHCVNS32-181/421-34 protease. Expression in Escherichia coli XL-1 Blue cells Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C
-
-
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Hepacivirus C A8DX91
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-
Hepacivirus C A8DXI7
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-
Hepacivirus C A8DXT5
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-
Hepacivirus C B0L492
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-
Hepacivirus C B0L493
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-
Hepacivirus C B0L494
-
-
Hepacivirus C B0L495
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-
Hepacivirus C B0L496
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-
Hepacivirus C B0L497
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-
Hepacivirus C Q8JRC7
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hepatitis virus C polyprotein NS5A/NS5B + H2O 123% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 165% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 23.5% catalytic efficiency Hepacivirus C ?
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?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 38.4% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 39.1% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 53.2% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 58.8% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 68.4% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 70.6% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 72.6% catalytic efficiency Hepacivirus C ?
-
?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 73.9% catalytic efficiency Hepacivirus C ?
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?
Hepatitis virus C polyprotein NS5A/NS5B + H2O 86.5% catalytic efficiency Hepacivirus C ?
-
?

Synonyms

Synonyms Comment Organism
NS3/4A protease
-
Hepacivirus C

Cofactor

Cofactor Comment Organism Structure
additional information enhancement of NS3 catalytic efficiency by the NS4A cofactor depends on a few specific amino acid residues Hepacivirus C