Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.98 extracted from

  • Shiryaev, S.A.; Ratnikov, B.I.; Chekanov, A.V.; Sikora, S.; Rozanov, D.V.; Godzik, A.; Wang, J.; Smith, J.W.; Huang, Z.; Lindberg, I.; Samuel, M.A.; Diamond, M.S.; Strongin, A.Y.
    Cleavage targets and the D-arginine-based inhibitors of the West Nile virus NS3 processing proteinase (2006), Biochem. J., 393, 503-511.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.98

Protein Variants

Protein Variants Comment Organism
H51A less than 1% of wild-type activity, no autolytic conversion into mature enzyme West Nile virus

Inhibitors

Inhibitors Comment Organism Structure
alpha1-anitrypsin
-
 West Nile virus
Aprotinin
-
 West Nile virus
deca-D-Arg-NH2
-
 West Nile virus
dodeca-D-Arg-NH2
-
 West Nile virus
dodeka-D-Arg-NH2
-
 West Nile virus
hepta-D-Arg-NH2
-
 West Nile virus
hexa-D-Arg-NH2
-
 West Nile virus
nona-D-Arg-NH2 reduces infection in primary neurons West Nile virus
octa-D-Arg-NH2
-
 West Nile virus
undeca-D-Arg-NH2
-
 West Nile virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 Boc-RVRR-7-amido-4-methylcoumarin pH 8.0, 18°C West Nile virus
0.043
-
 Pyr-RTKR-7-amido-4-methylcoumarin pH 8.0, 18°C West Nile virus
0.146
-
 Z-RR-7-amido-4-methylcoumarin pH 8.0, 18°C West Nile virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, mature protein, SDS-PAGE West Nile virus

Organism

Organism UniProt Comment Textmining
West Nile virus
-
 recombinantenzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification autolytic conversion of 36 kDa NS2B-NS3 construct into the 30 kDa NS3 enzyme West Nile virus

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, autolytic conversion of 36 kDa NS2B-NS3 construct into the 30 kDa NS3 enzyme West Nile virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-RVRR-7-amido-4-methylcoumarin + H2O
-
 West Nile virus ?
-
 ?
additional information no substrate: Boc-AGPR-AMC, Boc-QAR-AMC, Boc-O-benzyl-EGR-pNA West Nile virus ?
-
 ?
myelin basic protein + H2O
-
 West Nile virus ?
-
 ?
Pyr-RTKR-7-amido-4-methylcoumarin + H2O
-
 West Nile virus ?
-
 ?
Z-RR-7-amido-4-methylcoumarin + H2O
-
 West Nile virus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, mature protein, SDS-PAGE West Nile virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.062
-
 Z-RR-7-amido-4-methylcoumarin pH 8.0, 18°C West Nile virus
0.073
-
 Boc-RVRR-7-amido-4-methylcoumarin pH 8.0, 18°C West Nile virus
43
-
 Pyr-RTKR-7-amido-4-methylcoumarin pH 8.0, 18°C West Nile virus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
 undeca-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.000001
-
 dodeka-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.000002
-
 deca-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.000006
-
 nona-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.000017
-
 octa-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.000026
-
 Aprotinin pH 8.0, 18°C West Nile virus
0.000041
-
 hepta-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.000478
-
 hexa-D-Arg-NH2 pH 8.0, 18°C West Nile virus
0.00059
-
 alpha1-antitrypsin pH 8.0, 18°C West Nile virus