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Literature summary for 3.4.21.97 extracted from

  • Tigue, N.; Matharu, P.J.; Roberts, N.A.; Mills, J.S.; Kay, J.; Rupp, R.
    Cloning, expression, and characterization of the proteinase from human herpesvirus 6 (1996), J. Virol., 70, 4136-4141.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.97

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Human betaherpesvirus 6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid
-
 Human betaherpesvirus 5
0.033
-
 4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid
-
 Human betaherpesvirus 6
0.3
-
 succinyl-RRYIKASEPPV-NH2
-
 Human betaherpesvirus 6
0.5
-
 succinyl-RRYIKASEPPV-NH2
-
 Human betaherpesvirus 5
1
-
 succinyl-RRILNASLAPE-NH2
-
 Human betaherpesvirus 5
1
-
 succinyl-YVKASEPLA-NH2
-
 Human betaherpesvirus 5
2
-
 succinyl-RRILNASLAPE-NH2
-
 Human betaherpesvirus 6
3
-
 succinyl-SYVKASVS-NH2
-
 Human betaherpesvirus 6
4
-
 succinyl-VVNAS-2-aminobutyryl-R-NH2
-
 Human betaherpesvirus 5
4
-
 succinyl-YVKASEPLA-NH2
-
 Human betaherpesvirus 6
9
-
 succinyl-SYVKASVS-NH2
-
 Human betaherpesvirus 5
10
-
 succinyl-VVNAS-2-aminobutyryl-R-NH2
-
 Human betaherpesvirus 6
11
-
 succinyl-LVNAS-2-aminobutyryl-EPT-NH2
-
 Human betaherpesvirus 5
16
-
 succinyl-LVNAS-2-aminobutyryl-EPT-NH2
-
 Human betaherpesvirus 6

Organism

Organism UniProt Comment Textmining
Human betaherpesvirus 5
-
-
-
Human betaherpesvirus 6
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Human betaherpesvirus 6

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid + H2O
-
 Human betaherpesvirus 6 4-(4'-dimethylaminophenazo)benzoic acid-RGVVNA + S-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid
-
 ?
4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid + H2O
-
 Human betaherpesvirus 5 ?
-
 ?
4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid + H2O
-
 Human betaherpesvirus 5 4-(4'-dimethylaminophenazo)benzoic acid-RGVVNA + Ser-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid
-
 ?
succinyl-LVNAS-2-aminobutyryl-Arg-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-LVNA + Ser-2-aminobutyryl-Arg-NH2
-
 ?
succinyl-LVNAS-2-aminobutyryl-EPT-NH2
-
 Human betaherpesvirus 6 succinyl-LVNA + S-2-aminobutyryl-EPT-NH2
-
 ?
succinyl-LVNAS-2-aminobutyryl-EPT-NH2 + H2O
-
 Human betaherpesvirus 5 ?
-
 ?
succinyl-LVNAS-2-aminobutyryl-EPT-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-LVNA + Ser-2-aminobutyryl-EPT-NH2 + H2O
-
 ?
succinyl-RRILNASLAPE-NH2
-
 Human betaherpesvirus 6 succinyl-RRILNA + SLAPE-NH2
-
 ?
succinyl-RRILNASLAPE-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-RRILNA + SLAPE-NH2
-
 ?
succinyl-RRYIKASEPPV-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-RRYIKA + SEPPV-NH2
-
 ?
succinyl-RRYIKASEPPV-NH2 + H2O
-
 Human betaherpesvirus 6 succinyl-RRYIKA + SEPPV-NH2
-
 ?
succinyl-SYVKASVS-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-SYVKA + SVS-NH2
-
 ?
succinyl-SYVKASVS-NH2 + H2O
-
 Human betaherpesvirus 6 succinyl-SYVKA + SVS-NH2
-
 ?
succinyl-VVNAS-2-aminobutyryl-R-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-VVNA + S-2-aminobutyryl-R-NH2
-
 ?
succinyl-VVNAS-2-aminobutyryl-R-NH2 + H2O
-
 Human betaherpesvirus 6 succinyl-VVNA + S-2-aminobutyryl-R-NH2
-
 ?
succinyl-YVKASEPLA-NH2 + H2O
-
 Human betaherpesvirus 5 succinyl-YVKA + SEPLA-NH2
-
 ?
succinyl-YVKASEPLA-NH2 + H2O
-
 Human betaherpesvirus 6 succinyl-YVKA + SEPLA-NH2
-
 ?

Subunits

Subunits Comment Organism
monomer SDS-PAGE Human betaherpesvirus 6

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4 21
-
 Human betaherpesvirus 6

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 21
-
 Human betaherpesvirus 6

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00833
-
 4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid
-
 Human betaherpesvirus 6
0.0133
-
 4-(4'-dimethylaminophenazo)benzoic acid-RGVVNAS-2-aminobutanoyl-RLN-5-[(2'-aminoethyl)-amino]-naphthalene-1-sulfonic acid
-
 Human betaherpesvirus 5
0.0167
-
 succinyl-SYVKASVS-NH2
-
 Human betaherpesvirus 5
0.0333
-
 succinyl-RRYIKASEPPV-NH2
-
 Human betaherpesvirus 5
0.0333
-
 succinyl-YVKASEPLA-NH2
-
 Human betaherpesvirus 5
0.05
-
 succinyl-VVNAS-2-aminobutyryl-R-NH2
-
 Human betaherpesvirus 6
0.0833
-
 succinyl-LVNAS-2-aminobutyryl-EPT-NH2
-
 Human betaherpesvirus 5
0.0833
-
 succinyl-RRYIKASEPPV-NH2
-
 Human betaherpesvirus 6
0.1
-
 succinyl-RRILNASLAPE-NH2
-
 Human betaherpesvirus 5
0.133
-
 succinyl-LVNAS-2-aminobutyryl-Arg-NH2
-
 Human betaherpesvirus 5
0.167
-
 succinyl-YVKASEPLA-NH2
-
 Human betaherpesvirus 6
0.217
-
 succinyl-RRILNASLAPE-NH2
-
 Human betaherpesvirus 6
0.217
-
 succinyl-SYVKASVS-NH2
-
 Human betaherpesvirus 6
0.7
-
 succinyl-LVNAS-2-aminobutyryl-EPT-NH2
-
 Human betaherpesvirus 6

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Human betaherpesvirus 6

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: little activity, pH 9.5: 50% of maximal activity Human betaherpesvirus 6