Literature summary for 3.4.21.97 extracted from

Tomasselli, A.G.; Paddock, D.J.; Curry, K.A.; Garlick, R.L.; Leone, J.W.; Lull, J.M.; Mutchler, V.T.; Baker, C.A.; Cavey, G.S.; Methews, W.R.; Shelly, J.A.; Finzel, B.C.; Baldwin, E.T.; Wells, P.A.; Tomich, C.S.C.
Recombinant human cytomegalovirus protease with a C-terminal (His)6 extension: purification, autocatalytic release of the mature enzyme, and biochemical characterization (1996), Protein Expr. Purif., 14, 343-352.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Human betaherpesvirus 5

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Human betaherpesvirus 5

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.411	-	SYVKASVSPE	inclusion bodies	Human betaherpesvirus 5
0.462	-	RGVVNASSRLAK	inclusion bodies	Human betaherpesvirus 5
0.602	-	SYVKASVSPE	soluble enzyme	Human betaherpesvirus 5
0.758	-	RGVVNASSRLAK	soluble enzyme	Human betaherpesvirus 5

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28098	-	2 * 28098, electrospray mass spectrometry	Human betaherpesvirus 5
55000	-	gel filtration	Human betaherpesvirus 5

Organism

Organism	UniProt	Comment	Textmining
Human betaherpesvirus 5	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant forms	Human betaherpesvirus 5

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
RGVVNASSRLAK + H2O	substrate mimicking M-site substrate	Human betaherpesvirus 5	RGVVNA + SSRLAK	-	?
SYVKASVSPE + H2O	substrate mimicking R-site substrate	Human betaherpesvirus 5	SYVKA + SVSPE	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 28098, electrospray mass spectrometry	Human betaherpesvirus 5

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.06	-	SYVKASVSPE	soluble enzyme	Human betaherpesvirus 5
0.075	-	SYVKASVSPE	inclusion bodies	Human betaherpesvirus 5
0.28	-	RGVVNASSRLAK	soluble enzyme	Human betaherpesvirus 5
0.308	-	RGVVNASSRLAK	inclusion bodies	Human betaherpesvirus 5

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Release 2023.1 (January 2023)