Literature summary for 3.4.21.97 extracted from

Brignole, E.J.; Gibson, W.
Enzymatic activities of human cytomegalovirus maturational protease assemblin and its precursor (pPR, pUL80a) are comparable: [corrected] maximal activity of pPR requires self-interaction through its scaffolding domain (2007), J. Virol., 81, 4091-4103.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	self-interaction of pPR through its scaffolding domain plays an important role in activating the enzyme	Human betaherpesvirus 5

Cloned(Commentary)

Cloned (Comment)	Organism
into the NdeI and HindIII sites of pET-17b vector and expressed in Escherichia coli BL21(DE3)pLysS	Human betaherpesvirus 5

Protein Variants

Protein Variants	Comment	Organism
additional information	IC-assemblin and IC-assemblinHis mutants of assemblin, blocked for internal and cryptic site cleavage. Enzymatic activities of pPR mutants are indistinguishable from that of IC-assemblin	Human betaherpesvirus 5

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	IC-assemblin, Western immunoassay	Human betaherpesvirus 5

Organism

Organism	UniProt	Comment	Textmining
Human betaherpesvirus 5	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partially purified by immobilized metal affinity chromatography	Human betaherpesvirus 5

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
simian cytomegalovirus precursor assembly protein + H2O	assemblin and its precursor, pPR, have comparable enzymatic activities	Human betaherpesvirus 5	assembly protein	-	?

Synonyms

Synonyms	Comment	Organism
human cytomegalovirus maturational protease	-	Human betaherpesvirus 5

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Release 2023.1 (January 2023)