Literature summary for 3.4.21.96 extracted from

Hebert, E.M.; Mamone, G.; Picariello, G.; Raya, R.R.; Savoy, G.; Ferranti, P.; Addeo, F.
Characterization of the pattern of alphas1- and beta-casein breakdown and release of a bioactive peptide by a cell envelope proteinase from Lactobacillus delbrueckii subsp. lactis CRL 581 (2008), Appl. Environ. Microbiol., 74, 3682-3689.

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Inhibitors

Inhibitors	Comment	Organism	Structure
alpha-casein	maximum activity is observed in a basal minimal defined medium, whereas in the presence of alpha-casein CEP activity is inhibited 1fold	Lactobacillus delbrueckii
beta-casein	maximum activity is observed in a basal minimal defined medium, whereas in the presence of beta-casein CEP activity is inhibited 1.2fold	Lactobacillus delbrueckii
branched-chain amino acids	maximum activity is observed in a basal minimal defined medium, whereas in the presence of branched-chain amino acids CEP activity is inhibited 1.7fold	Lactobacillus delbrueckii
casamino acid	maximum activity is observed in a basal minimal defined medium, whereas in the presence of casamino acid 1% (w,v) or 0.2% (w,v) CEP activity is inhibited 70fold or 20fold, respectively	Lactobacillus delbrueckii
casitone	maximum activity is observed in a basal minimal defined medium, whereas in the presence of casitone 1% (w,v) or 2% (w,v) CEP activity is inhibited 99fold or 51fold, respectively	Lactobacillus delbrueckii
glycerol	maximum activity is observed in a basal minimal defined medium, whereas in the presence of glycerol CEP activity is inhibited 1.3fold	Lactobacillus delbrueckii
leucylglycylglycine	maximum activity is observed in a basal minimal defined medium, whereas in the presence of leucylglycylglycine 1 mM or 5 mM, CEP activity is inhibited 6.5fold or 12.9fold, respectively	Lactobacillus delbrueckii
leucylleucine	maximum activity is observed in a basal minimal defined medium, whereas in the presence of leucylleucine 1 mM or 5 mM, CEP activity is inhibited 7.2fold or 15.1fold, respectively	Lactobacillus delbrueckii
leucylproline	maximum activity is observed in a basal minimal defined medium, whereas in the presence of leucylproline 1 mM or 5 mM, CEP activity is inhibited 4fold or 8.8fold, respectively	Lactobacillus delbrueckii
low-molecular-mass peptides	maximum activity is observed in a basal minimal defined medium, whereas in the presence of low-molecular-mass peptides 1% (w, u) CEP activity is inhibited 99fold	Lactobacillus delbrueckii
NaCl	maximum activity is observed in a basal minimal defined medium, whereas in the presence of NaCl 0.25%-1% (w, u) CEP activity is inhibited 1.4fold	Lactobacillus delbrueckii
prolylleucine	maximum activity is observed in a basal minimal defined medium, whereas in the presence of prolylleucine 1 mM or 5 mM, CEP activity is inhibited 3.5fold or 9.9fold, respectively	Lactobacillus delbrueckii
yeast extract	maximum activity is observed in a basal minimal defined medium, whereas in the presence of yeast extract CEP activity is inhibited 68fold	Lactobacillus delbrueckii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii	-	subsp. lactis CRL 581	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	the CEP activity levels are controlled by the peptide content of the growth medium. The maximum activity is observed in a basal minimal defined medium	Lactobacillus delbrueckii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O	-	Lactobacillus delbrueckii	?	-	?

Synonyms

Synonyms	Comment	Organism
CEP	-	Lactobacillus delbrueckii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	assay at	Lactobacillus delbrueckii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Lactobacillus delbrueckii

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Release 2023.1 (January 2023)