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Literature summary for 3.4.21.96 extracted from

  • Broadbent, J.R.; Rodriguez, B.T.; Joseph, P.; Smith, E.A.; Steele, J.L.
    Conversion of Lactococcus lactis cell envelope proteinase specificity by partial allele exchange (2006), J. Appl. Microbiol., 100, 1307-1317.
    View publication on PubMed

Application

Application Comment Organism
biotechnology conversion of lactocepin substrate binding regions by allele exchange can effectively alter lactocepin specificity in industrial strains of Lactococcus lactis without significantly affecting other important strain properties. The methodology can be used to alter lactocepin specificity in commercial starter cultures with a propensity for bitter flavour defect Lactococcus lactis
industry method to alter lactocepin specificity in commercial starter cultures with a propensity for bitter flavour defect. PrtP derivatives developed by this approach should be suitable for commercial applications in the US and other countries with a favourable regulatory climate Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
additional information prtP replacement mutants (strains S3 or S4 with altered lactocepin gene sequence) have a slower rate of acidification than parental strains after 2.5 or 5 h incubation in ultra high temperature skim milk Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
commercial cheese starter culture, group h lactocepin
-
Lactococcus lactis Q49SG9
-
-
Lactococcus lactis Q49SH0
-
-
Lactococcus lactis s4
-
commercial cheese starter culture, group h lactocepin
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha(S1)-casein + H2O
-
Lactococcus lactis ?
-
?
alphas1-Casein fragment 1-23 + H2O
-
Lactococcus lactis Fragments of alphas1-casein fragment 1-23
-
?
alphas1-Casein fragment 1-23 + H2O
-
Lactococcus lactis s4 Fragments of alphas1-casein fragment 1-23
-
?

Synonyms

Synonyms Comment Organism
cell envelope proteinase
-
Lactococcus lactis